Electrochemical detection and analysis of tumor-derived extracellular vesicles to evaluate malignancy of pancreatic cystic neoplasm using integrated microfluidic device

微流控 液体活检 胰腺癌 材料科学 恶性肿瘤 生物医学工程 胞外囊泡 循环肿瘤细胞 纳米技术 癌症生物标志物 癌症 化学 病理 微泡 医学 转移 内科学 生物化学 小RNA 基因
作者
N.G. Gurudatt,Hogyeong Gwak,Kyung‐A Hyun,Seeun Jeong,Kyungyeon Lee,Sunyoung Park,Moon Jae Chung,Seong-Eun Kim,Jung Hyun Jo,Hyo‐Il Jung
出处
期刊:Biosensors and Bioelectronics [Elsevier BV]
卷期号:226: 115124-115124 被引量:4
标识
DOI:10.1016/j.bios.2023.115124
摘要

Tumor-derived extracellular vesicles (tdEVs) are one of the most promising biomarkers for liquid biopsy-based cancer diagnostics, owing to the expression of specific membrane proteins of their cellular origin. The investigation of epithelial-to-mesenchymal transition (EMT) in cancer using tdEVs is an alternative way of evaluating the risk of malignancy transformation. An ultra-sensitive selection and detection methodology is an essential step in developing a tdEVs-based cancer diagnostic device. In this study, we developed an indium-tin-oxide (ITO) sensor integrated microfluidic device consisting of two main parts: 1) a multi-orifice flow-fractionation (MOFF) channel for extraction of pure EVs by removing blood cellular debris, and 2) an ITO sensor coupled with a geometrically activated surface interaction (GASI) channel for enrichment and quantification of tdEV. The microfluidic channel and the ITO sensors are assembled with a 3D printed magnetic housing to prevent sample leakage and to easily attach/detach the sensors to/from the microfluidic channel. The tdEVs were successfully captured on the specific antibody modified ITO surfaces in the integrated microfluidic channel. The integrated sensors showed an excellent linear response between 103 and 109 tdEVs/mL. Simultaneous evaluation of the epithelial and mesenchymal markers on the tdEV surfaces successfully revealed the EMT index of the corresponding pancreatic cancer cells. Our ITO sensor integrated microfluidic device showed excellent detection in the clinically relevant tdEVs-concentration range for patients with pancreatic cystic neoplasms. Hence, this system is expected to open a new avenue for liquid biopsy-based cancer prognostics and diagnostics.
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