Human embryonic stem cell-specific role of YAP in maintenance of self-renewal and survival

下调和上调 胚胎干细胞 细胞生物学 生物 强力霉素 干细胞 细胞 免疫学 遗传学 基因 抗生素
作者
Mu Seog Choe,Chang Min Bae,So Jin Kim,Seung Tack Oh,Yu Jin Kown,Wonyoung Choi,Ho Jae Han,Kyung Min Baek,Woochul Chang,Joong‐Sun Kim,Kyung Seob Lim,Seung Pil Yun,Min Young Lee
出处
期刊:Cellular and Molecular Life Sciences [Springer Nature]
卷期号:79 (11) 被引量:2
标识
DOI:10.1007/s00018-022-04558-x
摘要

Human embryonic stem cells (hESCs) have unique characteristics, such as self-renewal and pluripotency, which are distinct from those of other cell types. These characteristics of hESCs are tightly regulated by complex signaling mechanisms. In this study, we demonstrate that yes-associated protein (YAP) functions in an hESC-specific manner to maintain self-renewal and survival in hESCs. hESCs were highly sensitive to YAP downregulation to promote cell survival. Interestingly, hESCs displayed dynamic changes in YAP expression in response to YAP downregulation. YAP was critical for the maintenance of self-renewal. Additionally, the function of YAP in maintenance of self-renewal and cell survival was hESC-specific. Doxycycline upregulated YAP in hESCs and attenuated the decreased cell survival induced by YAP downregulation. However, decreased expression of self-renewal markers triggered by YAP downregulation and neural/cardiac differentiation were affected by doxycycline treatment. Collectively, the results reveal the mechanism underlying the role of YAP and the novel function of doxycycline in hESCs.
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