Substitution of microalgae by bioflocs as a food source for the brine shrimp Artemia franciscana

Aquaculture ResearchVolume 53, Issue 12 p. 4374-4387 ORIGINAL ARTICLE Substitution of microalgae by bioflocs as a food source for the brine shrimp Artemia franciscana Elaheh Hasan-Nataj-Niazi, Corresponding Author Elaheh Hasan-Nataj-Niazi [email protected] Artemia and Aquaculture Research Institute, Urmia University, Urmia, Iran Department of Animal Sciences and Aquatic Ecology, Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Ghent, Belgium Correspondence Elaheh Hasan-Nataj-Niazi, Campus Coupure, F, Coupure Links 653, B-9000 Gent, Belgium. Email: [email protected] Farzaneh Noori, Artemia and Aquaculture Research Institute, Urmia University, Urmia, Iran. Email: [email protected]Search for more papers by this authorNaser Agh, Naser Agh Artemia and Aquaculture Research Institute, Urmia University, Urmia, IranSearch for more papers by this authorFarzaneh Noori, Corresponding Author Farzaneh Noori [email protected] Artemia and Aquaculture Research Institute, Urmia University, Urmia, Iran Correspondence Elaheh Hasan-Nataj-Niazi, Campus Coupure, F, Coupure Links 653, B-9000 Gent, Belgium. Email: [email protected] Farzaneh Noori, Artemia and Aquaculture Research Institute, Urmia University, Urmia, Iran. Email: [email protected]Search for more papers by this authorBehrooz Atashbar, Behrooz Atashbar orcid.org/0000-0002-6506-8597 Artemia and Aquaculture Research Institute, Urmia University, Urmia, IranSearch for more papers by this authorGilbert Van Stappen, Gilbert Van Stappen Department of Animal Sciences and Aquatic Ecology, Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Ghent, BelgiumSearch for more papers by this author Elaheh Hasan-Nataj-Niazi, Corresponding Author Elaheh Hasan-Nataj-Niazi [email protected] Artemia and Aquaculture Research Institute, Urmia University, Urmia, Iran Department of Animal Sciences and Aquatic Ecology, Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Ghent, Belgium Correspondence Elaheh Hasan-Nataj-Niazi, Campus Coupure, F, Coupure Links 653, B-9000 Gent, Belgium. Email: [email protected] Farzaneh Noori, Artemia and Aquaculture Research Institute, Urmia University, Urmia, Iran. Email: [email protected]Search for more papers by this authorNaser Agh, Naser Agh Artemia and Aquaculture Research Institute, Urmia University, Urmia, IranSearch for more papers by this authorFarzaneh Noori, Corresponding Author Farzaneh Noori [email protected] Artemia and Aquaculture Research Institute, Urmia University, Urmia, Iran Correspondence Elaheh Hasan-Nataj-Niazi, Campus Coupure, F, Coupure Links 653, B-9000 Gent, Belgium. Email: [email protected] Farzaneh Noori, Artemia and Aquaculture Research Institute, Urmia University, Urmia, Iran. Email: [email protected]Search for more papers by this authorBehrooz Atashbar, Behrooz Atashbar orcid.org/0000-0002-6506-8597 Artemia and Aquaculture Research Institute, Urmia University, Urmia, IranSearch for more papers by this authorGilbert Van Stappen, Gilbert Van Stappen Department of Animal Sciences and Aquatic Ecology, Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Ghent, BelgiumSearch for more papers by this author First published: 11 June 2022 https://doi.org/10.1111/are.15936 Funding information There is no fund for this research project. The authors confirm that they are not employed by the government of Iran or are not preparing articles in their 'personal capacity' (in other words, 'not as an official representative or on behalf of a sanctioned government'), and are employed at an academic or research institution where research or education is the primary function of the entity. Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Abstract This research was done to investigate the application of halophilic bacteria in a biofloc production system to grow Artemia franciscana in the laboratory, in which part of a standard microalgae feeding regime was replaced by bioflocs. Bioflocs were produced according to a standard procedure, using rice bran and vinasse as carbon sources, a commercial probiotic product, and halophilic Bacillus sp., originating from Urmia Lake, Iran, as halophilic bacteria. Three successive laboratory culture tests were conducted at salinities 60 (experiments 1 and 3) and 120 g L−1 (experiment 2) for 21, 14 and 14 days, respectively. Five feeding regimes were examined; the control treatment feeding a standard regime of only the microalga Dunaliella tertiolecta, and four biofloc treatments: 90% and 75% replacement of microalgae by bioflocs, both without and with halophilic addition. Depending on the experiment, Artemia performance was assessed as growth, reproductive performance, fatty acid and proximate composition and/or digestive enzyme activity. Applying halophilic bacteria in biofloc systems had no significant effect on the measured parameters. We further conclude that Artemia franciscana can be cultured successfully using bioflocs as the main food source, with limited microalgae supplementation (10%–25%) with no significant effect on survival, length, proximate composition and levels of most fatty acids in Artemia biomass. A number of fecundity parameters were negatively affected by 75% substitution of microalgae by bioflocs. This research opens interesting perspectives to produce Artemia at a bigger scale, e.g., in tanks or pond conditions, where production of sufficient quantities of suitable microalgae species might be problematic. CONFLICT OF INTEREST The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. Open Research DATA AVAILABILITY STATEMENT All relevant data are available from the authors upon request. Volume53, Issue12August 2022Pages 4374-4387 RelatedInformation