Construction of Nano-Carriers Coated with Platelet Membrane and Its Application in Targeted Therapy of Inflammation

In this study, poly (lactic acid-glycolic acid) (PLGA) nanoparticles loaded with anti-inflammatory drug ketoprofen (KET) were prepared and then coated with platelet membrane (PLTM) to form KET@PLTM-PLGA nano-particles (NPs). The particle size of the KET@PLTM-PLGA NPs is 176[Formula: see text]nm and the surface protein is the same as that of PLTM. The results of confocal microscopy and flow cytometry showed that the KET@PLTM-PLGA NPs uptake of RAW264.7 induced by LPS was significantly higher than that of KET@PLGA NPs, without PLTM, which was due to the binding of P-selectin to CD44 receptors on the surface of RAW264.7 cells induced by LPS on the surface of PLTM. Compared with other KET preparations, KET@PLTM-PLGA NPs have better anti-inflammatory effect.