自愈水凝胶
丝素
结冷胶
组织工程
生物相容性
硫酸软骨素
生物材料
细胞外基质
生物医学工程
化学
软骨
材料科学
糖胺聚糖
丝绸
透明质酸
化学工程
纳米纤维
软骨发生
纳米技术
高分子化学
生物化学
解剖
复合材料
有机化学
工程类
医学
食品科学
作者
Seongwon Lee,Joo Hee Choi,Jina Youn,Young-Hun Lee,Wooyoup Kim,Seungho Choe,Jeongeun Song,Rui L. Reis,Gilson Khang
出处
期刊:Biomolecules
[MDPI AG]
日期:2021-08-11
卷期号:11 (8): 1184-1184
被引量:25
摘要
Hydrogel is in the spotlight as a useful biomaterial in the field of drug delivery and tissue engineering due to its similar biological properties to a native extracellular matrix (ECM). Herein, we proposed a ternary hydrogel of gellan gum (GG), silk fibroin (SF), and chondroitin sulfate (CS) as a biomaterial for cartilage tissue engineering. The hydrogels were fabricated with a facile combination of the physical and chemical crosslinking method. The purpose of this study was to find the proper content of SF and GG for the ternary matrix and confirm the applicability of the hydrogel in vitro and in vivo. The chemical and mechanical properties were measured to confirm the suitability of the hydrogel for cartilage tissue engineering. The biocompatibility of the hydrogels was investigated by analyzing the cell morphology, adhesion, proliferation, migration, and growth of articular chondrocytes-laden hydrogels. The results showed that the higher proportion of GG enhanced the mechanical properties of the hydrogel but the groups with over 0.75% of GG exhibited gelling temperatures over 40 °C, which was a harsh condition for cell encapsulation. The 0.3% GG/3.7% SF/CS and 0.5% GG/3.5% SF/CS hydrogels were chosen for the in vitro study. The cells that were encapsulated in the hydrogels did not show any abnormalities and exhibited low cytotoxicity. The biochemical properties and gene expression of the encapsulated cells exhibited positive cell growth and expression of cartilage-specific ECM and genes in the 0.5% GG/3.5% SF/CS hydrogel. Overall, the study of the GG/SF/CS ternary hydrogel with an appropriate content showed that the combination of GG, SF, and CS can synergistically promote articular cartilage defect repair and has considerable potential for application as a biomaterial in cartilage tissue engineering.
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