体内
核酸
免疫佐剂
癌症研究
材料科学
癌症
免疫系统
纳米技术
免疫疗法
医学
化学
免疫学
生物
生物技术
生物化学
内科学
作者
Tongren Yang,Dong Huang,Chunhui Li,Deyao Zhao,Junshi Li,Mengjie Zhang,Yufeng Chen,Qining Wang,Zicai Liang,Xing‐Jie Liang,Zhihong Li,Yuanyu Huang
出处
期刊:Nano Today
[Elsevier]
日期:2021-02-01
卷期号:36: 101017-101017
被引量:40
标识
DOI:10.1016/j.nantod.2020.101017
摘要
Topical administration of siRNA, a clinically approved promising therapeutic modality, represents a much more transformative approach for drug development, compared to intravenous injection. To implement an efficient and extensive siRNA therapy in vivo, we engineered a rolling microneedle electrode array (RoMEA), which utilizes parallel circular blades with microneedle arrays on edge as electrodes. RoMEA integrates close-spaced microneedle electrodes and rolling structure to allow low-damage and large-area siRNA transfection. Upon applying RoMEA, regular micropores were established, efficient siRNA delivery and gene silence were achieved. In addition, employments of siRNA targeting programmed death-ligand 1 (PD-L1) alone, or combined with anti-programmed death-1 (PD-1) antibody or immunoadjuvant of CpG2395, in two tumor xenograft murine models demonstrated that proposed strategies restored efficient T cell immune response, conferring significant tumor growth inhibition with excellent safety profiles. RoMEA constitutes an unprecedented and ingenious clinic solution to large-area local delivery of nucleic acid for cancer immunotherapy.
科研通智能强力驱动
Strongly Powered by AbleSci AI