The medicinal active ingredients and their associated key enzyme genes are differentially regulated at different growth stages in Cornus officinalis and Cornus controversa

罗格宁 生物 香叶醇 官房 多年生植物 植物 传统医学 化学 精油 高效液相色谱法 医学 色谱法
作者
Jingjing Yang,Bo Cao,Ying Xue,Hui Liang,Yongmei Wu,Niping Zhao,Danni Hu,Pufan Gao,Guishuang Li,Chen Bai
出处
期刊:Industrial Crops and Products [Elsevier BV]
卷期号:142: 111858-111858 被引量:6
标识
DOI:10.1016/j.indcrop.2019.111858
摘要

Revealing the dynamic accumulation and synthesizing mechanism of medicinal active ingredients will be of great significance for the evaluation and utilization of crops in medicinal industry. Dogwood (Cornus officinalis Sizb. et Zucc.), a perennial deciduous tree in the Cornaceae family, possesses important medicinal values in protecting nerves, treating diabetes and kidney diseases, and preventing inflammation and apoptosis. Dengtaishu (Cornus controversa Hemsl.) is another perennial tree in the Cornaceae family. As a Cornus plant that is phylogenetically close to C. officinalis, it is widely used as horticultural landscape plants. Revealing the difference of active ingredients between the two plants within the same genus as well as its molecular mechanism will help improve resource utilization of medicinal crops. In this study, a systematic analysis on the fruit characters, content of active ingredients and expression of key enzyme genes of C. officinalis and C. controversa was conducted. First, the main fruit characters and active ingredients in fruits and leaves of C. officinalis and C. controversa during different growth stages were determined and analyzed. The key enzyme genes geraniol 10-dehydrogenase (G10H) and secologanin synthase (SLS) in the loganin synthesis pathway of C. officinalis and C. controversa were cloned, and the expression level of G10H and SLS was determined by quantitative real-time PCR (qRT-PCR) in leaves and fruits of the two species at different growth stages. Finally, correlation analysis between the content of loganin and the expression of G10H and SLS was conducted to reveal the possible molecular mechanism of loganin synthesis. The results showed that: (1) The fruit length, seed length, and 100-fruit weight of C. officinalis were significantly higher than those of C. controversa (P < 0.01). (2) The content of total sugar and cornel iridoid glycosides (CIGs) in fruits were significantly higher than those in leaves in both the two species, and they increased significantly with the duration of growth period. (3) The content of six active ingredients in leaves was significantly lower than that in fruits of the two species. Except for 5-hydroxymethylfurfural, the content of other five active ingredients in fruits of C. officinalis was significantly higher than that of C. controversa. (4) The content of loganin, cornuside, and protocatechuate in leaves of C. controversa were significantly higher than that of fruits of C. officinalis. (5) G10H was mainly expressed in the fruits of two species, and the expression in fruits of C. officinalis was significantly higher than that of C. controversa. The expression of SLS in fruits and leaves of C. controversa was significantly higher than that of C. officinalis at late growth stage. (6) Correlation analysis between loganin content and gene expression indicated that there was a positive correlation between the expression of G10H and the content of loganin, while the expression of SLS was negatively correlated with the content of loganin. These results first raise the possibility that C. controversa can be used as an alternative to C. officinalis in resource utilization. Meanwhile, the best harvest time with best quality and high yield for C. officinalis and C. controversa was determined. Furthermore, it was speculated that the significant differences of the expression of G10H and SLS in loganin synthesis pathway might be the main reason for the differences of active ingredients between the two species within the same genus. Taken together, these results will further provide insights into the improvement of both the quality and the yield of medicinal crops using approaches of germplasm breeding and genetic engineering.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
BettyNie完成签到 ,获得积分10
2秒前
优雅的平安完成签到 ,获得积分10
2秒前
冰糕完成签到,获得积分10
7秒前
9秒前
852应助完犊子采纳,获得10
13秒前
ruochenzu发布了新的文献求助10
14秒前
不想洗碗完成签到 ,获得积分10
16秒前
const完成签到,获得积分10
20秒前
hjx完成签到 ,获得积分10
20秒前
稳重的尔安完成签到,获得积分10
22秒前
量子星尘发布了新的文献求助10
23秒前
缓慢的饼干完成签到 ,获得积分10
23秒前
saturn完成签到 ,获得积分10
23秒前
金桔希子完成签到,获得积分10
24秒前
Breeze完成签到 ,获得积分10
24秒前
24秒前
昏睡的眼神完成签到 ,获得积分10
25秒前
文心同学完成签到,获得积分0
26秒前
demom完成签到 ,获得积分10
28秒前
duckspy完成签到 ,获得积分10
28秒前
完犊子发布了新的文献求助10
30秒前
普鲁卡因发布了新的文献求助30
31秒前
chenkj完成签到,获得积分10
33秒前
EricSai完成签到,获得积分10
33秒前
ikun完成签到,获得积分10
33秒前
李李发布了新的文献求助10
33秒前
章鱼小丸子完成签到 ,获得积分10
33秒前
byby完成签到,获得积分10
35秒前
THEO完成签到 ,获得积分10
36秒前
李健的小迷弟应助完犊子采纳,获得10
37秒前
38秒前
乐观的问兰完成签到 ,获得积分10
39秒前
书生完成签到,获得积分10
39秒前
朱佳宁完成签到 ,获得积分10
40秒前
Master完成签到 ,获得积分10
41秒前
怕孤独的香菇完成签到 ,获得积分10
41秒前
光亮冬寒完成签到,获得积分10
42秒前
FashionBoy应助李李采纳,获得10
43秒前
搬砖完成签到 ,获得积分10
44秒前
kingmp2完成签到 ,获得积分10
44秒前
高分求助中
【提示信息,请勿应助】关于scihub 10000
Les Mantodea de Guyane: Insecta, Polyneoptera [The Mantids of French Guiana] 3000
徐淮辽南地区新元古代叠层石及生物地层 3000
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
Handbook of Industrial Diamonds.Vol2 1100
Global Eyelash Assessment scale (GEA) 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 550
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4038112
求助须知:如何正确求助?哪些是违规求助? 3575788
关于积分的说明 11373801
捐赠科研通 3305604
什么是DOI,文献DOI怎么找? 1819255
邀请新用户注册赠送积分活动 892655
科研通“疑难数据库(出版商)”最低求助积分说明 815022