Changes of lipid metabolism related microRNAs expression in subclinical hypothyroidism mice model

Objective To obtain a non-invasive subclinical hypothyroidism (SCH) mouse model, and to explore microRNAs profile related to lipid metabolism in the model mice. Methods C57BL/6 male mice (8 weeks) were treated with methimazole (MMI, 0.08 mg/kg BW/d) to construct SCH mouse model. MicroRNAs profiling analysis was performed by real-time quantitative polymerase chain reaction (qRT-PCR). Results Compared with the control group, the serum thyroid stimulating hormone (TSH) in subclinical hypothyroidism group increased significantly (P 0.05), which was in line with the diagnostic criteria of SCH. SCH mice was accompanied by dyslipidemia and liver lipid metabolism disorders. Four lipid metabolism related miRNAs, miR-33, miR-122, miR-199a-5p, and miR-375 in the liver of SCH mice were significantly decreased compared with those of control(P<0.05). Conclusion The noninvasive SCH model generated by MMI and miRNAs profile provide an animal model and a molecular basis for the study of SCH related lipid metabolism disorders. (Chin J Endocrinol Metab, 2018, 34: 410-415) Key words: Subclinical hypothyroidism; Methimazole; MicroRNAs; Lipid metabolism