亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Su1246 SPECIFIC BACTERIA ISOLATED FROM HUMAN BREAST MILK BOLSTER INTESTINAL EPITHELIAL BARRIER FUNCTION, AND AMELIORATE DYSFUNCTION INDUCED BY SALMONELLA ENTERICA INFECTION

莱菔硫烷 活力测定 化学 体内 细胞凋亡 雷苏林 微生物学 免疫学 分子生物学 生物 生物化学 生物技术
作者
Jasia Anjum,Andrew Quach,Elaine Hanson,Arsalan Zaidi,Muhammad Tariq,Kim E. Barrett
出处
期刊:Gastroenterology [Elsevier BV]
卷期号:158 (6): S-556
标识
DOI:10.1016/s0016-5085(20)32094-1
摘要

Background: Sulforaphane (SFN) is a plant derived glucosinolate activated from a precursor metabolite to an active isothiocyonate by myrosinase activity of gut microbiota.This phytochemical has numerous purported health benefits and has been extensively studied for anticarcinogenic effects.Recently, SFN's anti-inflammatory properties through NFK-B and Nrf2 pathways have stoked interest in exploring benefits of SFN for chronic inflammatory disease such as IBD.In vivo studies demonstrate SFN treatment ameliorates DSS colitis in mice.Further, In vitro work shows differential effects of SFN on proliferation and apoptosis of primary vs. transformed cells.To our knowledge, SFN has not yet been studied in human colonoid platform and we sought to examine SFN effects on colonoid viability in comparison to T84 cells, a metastatic colonic carcinoma cell line.Methods: T84 cells and human colonoids were passaged and treated at 48hrs with SFN 0, 5, 10, 20, and 40 uM concentrations.Cellular viability was measured using a commercial resazurin based redox reagent, with fluorescence measurements obtained at 24 and 48hrs.Colonoid measurements were normalized for background fluorescence created by the Matrigel dome.One-way ANOVAs were conducted to compare mean viability scores between treated and untreated T84 cells and colonoids at 24 and 48hrs.Post hoc comparisons were performed using Tukey HSD to examine concentration specific differences.Results: SFN treatment of T84 cells led to a significant effect on viability between groups (F (5, 142) = 10.53P<0.0001)), with increased relative fluorescent scores of 2363 for 10 uM and 2363 at 20 uM SFN vs 1726 in untreated controls (P=0.024and P=0.05, respectively).In contrast, we observed no significant viability differences between untreated controls and the 40uM concentration (p = 0.45).Similarly, in colonoids SFN had a significant effect on mean viability at both 24 and 48 hrs, F (4, 225)=2.474P=0.045.Dose dependent effects were most prominent at 24hrs with mean normalized fluorescence score 158.5 for 20 uM vs 101 for control (p=0.013) and trended towards significance in the 10 uM SFN group with score of 147.6 (p=0.08).However, no significant viability difference was observed between control and 40 uM concentration (p= 0.99).Conclusion: At low and physiologic concentrations of 10 and 20 uM SFN enhances intestinal cell viability in both T84 cells and human colonoids.At concentrations exceeding 40 uM, this proliferative effect is diminished.The mechanisms behind the dose dependent manner of SFN effects warrant further examination to better understand potential strategies for rational use of sulforaphane as a synbiotic therapy for IBD.Further, colonoids appear to be a useful platform for preclinical testing with SFN and may provide a platform to explore cell-specific mechanisms and effects on SFN.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
小刘完成签到,获得积分10
2秒前
8秒前
三笠完成签到,获得积分10
10秒前
传奇3应助Liiiiiiiiii采纳,获得10
12秒前
gt完成签到 ,获得积分10
22秒前
24秒前
文静栾完成签到 ,获得积分10
26秒前
芝麻关注了科研通微信公众号
39秒前
44秒前
所所应助徐悦月采纳,获得10
46秒前
46秒前
核桃发布了新的文献求助10
48秒前
53秒前
芝麻发布了新的文献求助10
54秒前
昏睡的沛柔完成签到 ,获得积分10
56秒前
Aliya完成签到 ,获得积分10
1分钟前
酷波er应助科研通管家采纳,获得10
1分钟前
1分钟前
1分钟前
1分钟前
李李发布了新的文献求助10
1分钟前
bksqc发布了新的文献求助10
1分钟前
要文献啊完成签到 ,获得积分10
1分钟前
1分钟前
WUHUIWEN完成签到,获得积分10
1分钟前
Liiiiiiiiii发布了新的文献求助10
1分钟前
1分钟前
1分钟前
尊敬夜南发布了新的文献求助10
1分钟前
韦雪莲完成签到 ,获得积分10
2分钟前
wz完成签到,获得积分10
2分钟前
2分钟前
Liiiiiiiiii完成签到,获得积分10
2分钟前
杜德爽发布了新的文献求助10
2分钟前
好好搞科研完成签到 ,获得积分10
2分钟前
2分钟前
热情的寄瑶完成签到 ,获得积分10
2分钟前
yanglinhai完成签到 ,获得积分10
2分钟前
饱满含玉完成签到,获得积分10
2分钟前
2分钟前
高分求助中
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3990031
求助须知:如何正确求助?哪些是违规求助? 3532108
关于积分的说明 11256334
捐赠科研通 3270943
什么是DOI,文献DOI怎么找? 1805146
邀请新用户注册赠送积分活动 882270
科研通“疑难数据库(出版商)”最低求助积分说明 809228