适体
检出限
化学
纳米技术
生物标志物
金属有机骨架
共价键
组合化学
分析物
色谱法
材料科学
生物化学
有机化学
分子生物学
生物
吸附
作者
Ya Li,Zhiling Liu,Wenbo Lu,Man Zhao,He Xiao,Tianjun Hu,Jiwei Ma,Zhanfeng Zheng,Jianfeng Jia,Huang Wu
出处
期刊:Analyst
[The Royal Society of Chemistry]
日期:2021-01-01
卷期号:146 (3): 979-988
被引量:29
摘要
As one of the significant serum cytokines, platelet-derived growth factor-BB (PDGF-BB) is a crucial protein biomarker overexpressed in human life-threatening tumors, the sensitive identification and quantification of which are urgently desired but challenging. Herein we report a novel core-shell nanoarchitecture consisting of Cu-based metal-organic frameworks (Cu-MOFs) and covalent organic frameworks (denoted as TpBD-COFs), which was used to prepare an aptasensor for the detection of platelet-derived growth factor-BB (PDGF-BB). The central Cu-MOFs function as signal labels with no need for extra redox media, whereas the porous TpBD serves as the shell to immobilize the PDGF-BB-targeted aptamer strands in abundance via strong interactions involving π-π stacking, electrostatic, and hydrogen bonding interactions. The proposed aptasensor based on Cu-MOF@TpBD can achieve a detection limit as low as 0.034 pg mL-1 within the dynamic detection range from 0.0001 to 60 ng mL-1. The hybridization of MOFs and COFs, together with the immobilization with the specific analyte targeted aptamer, provides a promising and propagable approach to prepare an aptasensor for the simple, sensitive, and selective detection of a specific biomarker in clinical diagnosis.
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