酵母
己酸
生物化学
脂肪酸
代谢工程
细胞外
脂肪酸合酶
雅罗维亚
酿酒酵母
化学
生物
基因
作者
Jan Gajewski,Renata Pavlovic,M. Dominik Fischer,Eckhard Boles,Martin Grininger
摘要
Abstract Fatty acids (FAs) are considered strategically important platform compounds that can be accessed by sustainable microbial approaches. Here we report the reprogramming of chain-length control of Saccharomyces cerevisiae fatty acid synthase (FAS). Aiming for short-chain FAs (SCFAs) producing baker’s yeast, we perform a highly rational and minimally invasive protein engineering approach that leaves the molecular mechanisms of FASs unchanged. Finally, we identify five mutations that can turn baker’s yeast into a SCFA producing system. Without any further pathway engineering, we achieve yields in extracellular concentrations of SCFAs, mainly hexanoic acid (C 6 -FA) and octanoic acid (C 8 -FA), of 464 mg l −1 in total. Furthermore, we succeed in the specific production of C 6 - or C 8 -FA in extracellular concentrations of 72 and 245 mg l −1 , respectively. The presented technology is applicable far beyond baker’s yeast, and can be plugged into essentially all currently available FA overproducing microorganisms.
科研通智能强力驱动
Strongly Powered by AbleSci AI