Tunable Enzymatic Activity and Enhanced Stability of Cellulase Immobilized in Biohybrid Nanogels

纳米凝胶 化学 纤维素酶 乙二醇 分散性 聚合 共聚物 两亲性 链式转移 化学工程 高分子化学 聚合物 有机化学 组合化学 纤维素 自由基聚合 药物输送 工程类
作者
Huan Peng,Kristin Rübsam,Felix Jakob,Ulrich Schwaneberg,Andrij Pich
出处
期刊:Biomacromolecules [American Chemical Society]
卷期号:17 (11): 3619-3631 被引量:51
标识
DOI:10.1021/acs.biomac.6b01119
摘要

This paper reports a facile approach for encapsulation of enzymes in nanogels. Our approach is based on the use of reactive copolymers able to get conjugated with enzyme and build 3D colloidal networks or biohybrid nanogels. In a systematic study, we address the following question: how the chemical structure of nanogel network influences the biocatalytic activity of entrapped enzyme? The developed method allows precise control of the enzyme activity and improvement of enzyme resistance against harsh store conditions, chaotropic agents, and organic solvents. The nanogels were constructed via direct chemical cross-linking of water-soluble reactive copolymers poly(N-vinylpyrrolidone-co-N-methacryloxysuccinimide) with proteins such as enhanced green fluorescent protein (EGFP) and cellulase in water-in-oil emulsion. The water-soluble reactive copolymers with controlled amount of reactive succinimide groups and narrow dispersity were synthesized via reversible addition-fragmentation chain transfer (RAFT) polymerization. Poly(ethylene glycol) bis(3-aminopropyl) and branched polyethylenimine were utilized as model cross-linkers to optimize synthesis of nanogels with different architectures in the preliminary experiments. Biofluorescent nanogels with different loading amount of EGFP and varying cross-linking densities were obtained. We demonstrate that the biocatalytic activity of cellulase-conjugated nanogels (CNG) can be elegantly tuned by control of their cross-linking degrees. Circular dichroism (CD) spectra demonstrated that the secondary structures of the immobilized cellulase were changed in the aspect of α-helix contents. The secondary structures of cellulase in highly cross-linked nanogels were strongly altered compared with loosely cross-linked nanogels. The fluorescence resonance energy transfer (FRET) based study further revealed that nanogels with lower cross-linking degree enable higher substrate transport rate, providing easier access to the active site of the enzyme. The biohybrid nanogels demonstrated significantly improved stability in preserving enzymatic activity compared with free cellulase. The functional biohybrid nanogels with tunable enzymatic activity and improved stability are promising candidates for applications in biocatalysis, biomass conversion, or energy utilization fields.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
1秒前
qweerrtt完成签到,获得积分10
8秒前
8秒前
与共发布了新的文献求助10
9秒前
carly完成签到 ,获得积分10
10秒前
颢懿完成签到 ,获得积分10
13秒前
量子星尘发布了新的文献求助10
14秒前
16秒前
ljc完成签到 ,获得积分10
17秒前
Java完成签到,获得积分10
21秒前
23秒前
鲤鱼安青完成签到 ,获得积分10
25秒前
25秒前
dollarpuff完成签到 ,获得积分10
28秒前
28秒前
mmmmmMM完成签到,获得积分10
35秒前
luckweb完成签到,获得积分10
41秒前
猫的毛完成签到 ,获得积分10
42秒前
nicky完成签到 ,获得积分10
43秒前
麦子完成签到 ,获得积分10
44秒前
44秒前
Wilson完成签到 ,获得积分10
45秒前
luckweb发布了新的文献求助10
45秒前
45秒前
49秒前
52秒前
传奇3应助wujiwuhui采纳,获得10
54秒前
开心寄松完成签到,获得积分10
56秒前
北宫完成签到 ,获得积分10
56秒前
wansida完成签到,获得积分10
1分钟前
QXS完成签到 ,获得积分10
1分钟前
1分钟前
菠萝完成签到 ,获得积分10
1分钟前
领导范儿应助Villanellel采纳,获得10
1分钟前
wintersss完成签到,获得积分10
1分钟前
尹尹发布了新的文献求助10
1分钟前
量子星尘发布了新的文献求助10
1分钟前
zzzzzz完成签到 ,获得积分10
1分钟前
坦率的枕头完成签到,获得积分10
1分钟前
XS_QI完成签到 ,获得积分10
1分钟前
高分求助中
【提示信息,请勿应助】关于scihub 10000
Les Mantodea de Guyane: Insecta, Polyneoptera [The Mantids of French Guiana] 3000
徐淮辽南地区新元古代叠层石及生物地层 3000
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
Handbook of Industrial Diamonds.Vol2 1100
Global Eyelash Assessment scale (GEA) 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 550
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4038066
求助须知:如何正确求助?哪些是违规求助? 3575779
关于积分的说明 11373801
捐赠科研通 3305584
什么是DOI,文献DOI怎么找? 1819239
邀请新用户注册赠送积分活动 892655
科研通“疑难数据库(出版商)”最低求助积分说明 815022