Protective effects of mannan/β-glucans from yeast cell wall on the deoxyniyalenol-induced oxidative stress and autophagy in IPEC-J2 cells

The aim of this study was to investigate the effects of biomacromolecules mannan/β-glucans from yeast cell wall (BYCW) to alleviate Deoxynivalenol(DON)-induced injury. Considering that DON has strong oxidizing effect and stimulates autophagy and apoptosis, we examined the effects of BYCW on consequent oxidative stress damage indicators, cells autophagy and apoptosis induced by DON using the porcine jejunum epithelial cell lines (IPEC-J2) as a cell culture model. The results showed that application of BYCW could reverse the decrease of cell viability by DON significantly, and suppress the levels of tumor necrosis factor-α (TNF-α) and interleukin-8 and -6 (IL-8 and IL-6), except IL-1β. Further experiments revealed that BYCW treatment counteracted the DON-induced down-regulation of intracellular glutathione (GSH) and up-regulation of reactive oxygen species (ROS) and malondialdehyde (MDA). Through western blot analysis, we observed that BYCW treatment was able to down-regulate the expression of autophagy protein LC3-II and up-regulate the expression of P62 protein against DON, which suggested that autophagy induced by DON may be suppressed. Altogether, these results indicated a potential ability of supplementation of BYCW to improve cell growth and metabolism as well as the preventive properties of BYCW against the DON-induced cell damage by activating antioxidant system.