REDUCTION OF ENDOTHELIAL AND SMOOTH MUSCLE DENSITY IN THE CORPORA CAVERNOSA OF THE STREPTOZOTOCIN INDUCED DIABETIC RAT

No AccessJournal of UrologyINVESTIGATIVE UROLOGY1 Nov 2000REDUCTION OF ENDOTHELIAL AND SMOOTH MUSCLE DENSITY IN THE CORPORA CAVERNOSA OF THE STREPTOZOTOCIN INDUCED DIABETIC RAT T. BURCHARDT, M. BURCHARDT, J. KARDEN, R. BUTTYAN, A. SHABSIGH, A. de la TAILLE, P.Y. NG, A.G. ANASTASIADIS, and R. SHABSIGH T. BURCHARDTT. BURCHARDT , M. BURCHARDTM. BURCHARDT , J. KARDENJ. KARDEN , R. BUTTYANR. BUTTYAN , A. SHABSIGHA. SHABSIGH , A. de la TAILLEA. de la TAILLE , P.Y. NGP.Y. NG , A.G. ANASTASIADISA.G. ANASTASIADIS , and R. SHABSIGHR. SHABSIGH View All Author Informationhttps://doi.org/10.1016/S0022-5347(05)67111-XAboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: Erectile dysfunction is one of the most prevalent complications of diabetes in males. Because adequate vascular perfusion is needed for appropriate erectile tissue function a likely reason for the high incidence of this complication in diabetics is a pathological change associated with the disease in vascularization of erectile tissues. We investigate whether chronic diabetes may induce changes in vascularization of the corpora cavernosa using a computerized image analysis system to quantify changes in the smooth muscle and endothelial cell content of the corpora cavernosa of diabetic rats induced by streptozotocin 6 months previously, and compare these changes to those associated with aging. Materials and Methods: We studied 3 groups of rats, including 10-week-old untreated controls, diabetic rats treated with streptozotocin for 6 months starting at age 10 weeks and 18-month-old rats (aged). Penile shafts from these groups were excised, fixed, sectioned and immunostained with anti-smooth muscle actin to identify smooth muscle cells and anti-CD31 to identify endothelial cells. Computerized image analysis was used to quantify the percent area within the corpora cavernosa occupied by smooth muscle cells or endothelial cells, and the data were compared among the groups. Results: We identified a highly significant decrease in the percentage of smooth muscle and endothelial cells within the cavernosa areas of diabetic rats compared to control or aged rats. Mean cavernous smooth muscle cell content was 15.28 ± 2.54% in control rats and 9.83 ± 1.21% in diabetic rats (p = 0.0001). Likewise, cavernous endothelial cell content was 6.93 ± 0.86% in the control group and 4.01 ± 1.08% in the diabetic group (p = 0.0001). However, no statistical difference of smooth muscle or endothelial cell content was found between control and aged rats. 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Google Scholar From the Departments of Urology and Pathology, The College of Physicians and Surgeons of Columbia University, New York, New York, and Department of Urology, Heinrich-Heine-Universitaet, Duesseldorf, Germany© 2000 by American Urological Association, Inc.FiguresReferencesRelatedDetailsCited byBatbold D, Song K, Park J, Park S, Lee T, Ryu D, Suh Y, Kwon Y, Ryu J and Suh J (2018) Sac-1004, a Pseudo-Sugar Derivative of Cholesterol, Restores Erectile Function through Reconstruction of Nonleaky and Functional Cavernous Angiogenesis in the Streptozotocin Induced Diabetic MouseJournal of Urology, VOL. 195, NO. 6, (1936-1946), Online publication date: 1-Jun-2016.Safarinejad M, Shafiei N and Safarinejad S (2018) The Influence of Promoter –202 A/C Polymorphism (rs2854744) of the IGFBP-3 Gene on Erectile Dysfunction Risk and Serum Levels of IGF-I and IGFBP-3Journal of Urology, VOL. 189, NO. 1, (374-379), Online publication date: 1-Jan-2013.SUETOMI T, HISASUE S, SATO Y, TABATA Y, AKAZA H and TSUKAMOTO T (2018) EFFECT OF BASIC FIBROBLAST GROWTH FACTOR INCORPORATING GELATIN MICROSPHERES ON ERECTILE FUNCTION IN THE DIABETIC RATJournal of Urology, VOL. 173, NO. 4, (1423-1428), Online publication date: 1-Apr-2005.YAMANAKA M, SHIRAI M, SHIINA H, TANAKA Y, TSUJIMURA A, MATSUMIYA K, OKUYAMA A and DAHIYA R (2018) Diabetes Induced Erectile Dysfunction and Apoptosis in Penile Crura are Recovered by Insulin Treatment in RatsJournal of Urology, VOL. 170, NO. 1, (291-297), Online publication date: 1-Jul-2003.USER H, HAIRSTON J, ZELNER D, McKENNA K and McVARY K (2018) Penile Weight and Cell Subtype Specific Changes in a Post-Radical Prostatectomy Model of Erectile DysfunctionJournal of Urology, VOL. 169, NO. 3, (1175-1179), Online publication date: 1-Mar-2003. Volume 164Issue 5November 2000Page: 1807-1811 Advertisement Copyright & Permissions© 2000 by American Urological Association, Inc.Keywordsmuscle, smoothdiabetes mellitusendotheliumratsimpotenceMetricsAuthor Information T. BURCHARDT More articles by this author M. BURCHARDT More articles by this author J. KARDEN More articles by this author R. BUTTYAN More articles by this author A. SHABSIGH More articles by this author A. de la TAILLE More articles by this author P.Y. NG More articles by this author A.G. ANASTASIADIS More articles by this author R. SHABSIGH More articles by this author Expand All Advertisement PDF downloadLoading ...