生物塑料
合成气
化学
甲醇
羟基烷酸
代谢工程
原材料
二氧化碳
能源
生物量(生态学)
碳源
醋酸乙烯酯
有机化学
食品科学
生物化学
废物管理
细菌
催化作用
生物
酶
煤
农学
聚合物
工程类
共聚物
遗传学
作者
Jihee Yoon,Jiyun Bae,Sung-Jin Kang,Byung‐Kwan Cho,Min‐Kyu Oh
标识
DOI:10.1016/j.biortech.2022.127127
摘要
Acetate is regarded as a sustainable microbial feedstock that is synthesized from biowastes such as synthesis gas (syngas), carbon dioxide, lignocellulose, or organic waste. In this study, Methylorubrum extorquens AM1 was engineered to improve the production of bioplastic poly-3-hydroxybutyrate (PHB) using acetate as the sole carbon source. To utilize acetate as a carbon source and methanol as an energy source, acs encoding acetyl-CoA synthetase and fdh from Burkholderia stabilis were overexpressed, while ftfL involved in the assimilation of methanol into formyl-tetrahydrofolate was deleted. The yields of biomass and PHB from acetate significantly improved, and the growth rate and PHB content of the bacteria increased. In addition, sustainability of the PHB production was demonstrated using acetate derived from carbon dioxide and syngas. This study shows that biopolymers could be synthesized efficiently using acetate as the sole carbon source through metabolic engineering and the supply of energy cofactors.
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