Evaluation of Amino Acid and Vitamin B2 and E Composition of Genetically Modified Antidwarf Mosaic Maize by Automatic Amino acid Analyzer and HPLC

Transgenic technology plays an important role in increasing food production, improving nutrition and food processing and storage characteristics 1 .At the same time, the safety of the genetically modified foods are widely recognized 2 and nutrition assessment is an important part of safety evaluation 3 .But the nutrition change of transgenic food was paid less attention.Corn, as one of the world's most important food crops, is the important raw material of modern industry, food, pharmaceutical and chemical fields.Genetically modified antidwarf mosaic maize is an important technical means for resistance to mosaic virus, but the nutritional structure and chemical composition of genetically modified is still unclear.In this study, the amino acids and vitamins composition of genetically modified antidwarf mosaic maize and its original parents of non-genetically modified corn were comprehensively evaluated, which provides the basis for safety assessment of transgenic corn.Genetically modified maize dwarf mosaic resistance and non-genetically modified maize were gifted by Professor Sun Yi of Shanxi Agricultural Biotechnology Research Center, two cultivars were grown under the same conditions and management.Sampling by the five-point sampling method after maturity, dry at natural conditions, smash, screening through 40 mesh sieve, save in the vacuum bag.Three repeated measurements for each sample.