Toxin diversity revealed by de novo transcriptome assembly for venom gland in two species of spiders (Trichonephila clavata and Sinopoda pengi)

During long-term predator–prey coevolution, spiders have generated a vast diversity of toxins. Trichonephila clavata is a web-spinning spider whose large, well-constructed webs and venomous arsenal facilitate prey capture. In contrast, Sinopoda pengi is an ambush predator with agile locomotion and strong chelicerae for hunting. In this study, transcriptomic analysis was performed to describe the predicted toxins of S. pengi and T. clavata . A total of 43 and 47 of these unigenes from S. pengi and T. clavata , respectively, were predicted to have toxin activity. Putative neurotoxins were classified to the family level according to cysteine arrangement; 4 and 6 toxin families were produced by S. pengi and T. clavata , respectively. In addition, potential metalloproteases, acetylcholinesterases, serine proteases, hyaluronidases and phospholipases were found by annotation in databases. In summary, molecular templates with potential application value for medical and biological fields were obtained by classifying and characterizing presumed venom components, which established a foundation for further study of venom. • Predicted venom components in two species of spiders with different predatory habits by transcriptomic ( Sinopoda pengi and Trichonephila clavata ) (Fig 1). • Through transcriptome analysis, we obtained the toxin expression profiles of the two spiders, which will be helpful for the future analysis of toxin evolution and the development of medical and agricultural applications. • Putative neurotoxins were classified to the family level according to cysteine ar-rangement; 4 and 6 toxin families were produced by S. pengi and T. clavata , respec-tively. In addition, potential metalloproteases, acetylcholinesterases, serine proteases, hyaluronidases and phospholipases were found by annotation in databases.