Digging and identification of novel microorganisms from the soil environments with high methanol-tolerant lipase production for biodiesel preparation

脂肪酶 生物柴油 甲醇 嗜麦芽窄食单胞菌 三丁酸甘油酯 微生物 生物柴油生产 化学 生物燃料 食品科学 微生物学 生物 有机化学 生物技术 细菌 催化作用 铜绿假单胞菌 遗传学
作者
Zhiyong Tan,Gang Chen,Yipin Zhao,Hao Shi,Shiyan Wang,Muhammad Bilal,Li Dong,Xiangqian Li
出处
期刊:Environmental Research [Elsevier BV]
卷期号:212: 113570-113570 被引量:8
标识
DOI:10.1016/j.envres.2022.113570
摘要

Converting renewable biomass into carbon-neutral biofuels is one of the most effective strategies to achieve zero carbon emissions and contribute to environmental protection. Microorganisms from the soil were primarily screened on the rhodamine B-plate for highly-active lipase producing strains and re-screened on a tributyrin-methanol plate using crude lipases produced from the initially screened-out strains. The lipase-producing strains with higher methanol-tolerant lipase were identified based on morphological characteristics and 16S rDNA sequencing. The crude lipases with much higher methanol-tolerance from screened top-4 strains, Stenotrophomonas maltophilia D18, Lysinibacillus fusiformis B23, Acinetobacter junii C69, and A. pittii C95 showed temperature optima of 25 °C, 35 °C, 30 °C, and 30 °C at pH 7.0, respectively, while their pH optima were 8.0, 7.0, 7.5, and 7.5 at each optimum temperature, respectively. After 24-h incubation, they retained more than 85% of their original activities in 25%, 15%, 20%, and 20% of methanol, respectively. They catalyzed the conversion of soybean oil into biodiesel by yields of 63.1%, 35.4%, 74.6%, and 78.5% after 24-h reactions, respectively. In conclusion, the as-isolated microorganisms producing high methanol-tolerant lipase are considered promising to provide robust biocatalyst for efficient biodiesel preparation and other industrial applications.
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