信息素
生物
转录组
性信息素
基因
生物合成
还原酶
过氧化物酶体
醛氧化酶
生物化学
基因表达
酶
遗传学
黄嘌呤氧化酶
作者
Wook Hyun,Chung Ryul Jung,Yoon-Jeong Hwang,Dae‐Weon Lee
标识
DOI:10.1016/j.aspen.2017.09.008
摘要
The pheromone biosynthesis in Plutella xylostella is more active in the scotophase than that of the photophase, indicating that there may be changes of gene expression in the pheromone gland according to the photoperiod. To identify genes contributing to change in pheromone production, we analyzed transcriptomes of pheromone glands from both decapitated females (PG-minus) in the photophase and normal ones (PG-plus) in the scotophase. Deep sequencing for mRNAs in the pheromone gland yielded approximately 7.5 Gb and 10,303 transcripts showing positive FPKM value were analyzed. Differentially expressed gene analysis revealed that up- and down-regulated transcripts were 310 and 326 in the PG-plus transcriptome, respectively. Genes putatively involved in the pheromone biosynthesis pathway were identified such as acetyl-CoA carboxylase, acetyl-CoA dehydrogenase, fatty acid synthase (FAS), desaturases (Δ9 and Δ 11) and fatty acid reductases of pheromone gland (pgFAR), alcohol oxidase, aldehyde oxidase and aldehyde reductase, etc. Quantitative RT-PCR revealed that expressions of FAS, Δ11 desaturase and pgFAR were significantly higher in PG-plus, suggesting that they may have crucial roles in sex pheromone biosynthesis of P. xylostella.
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