Stress relaxing hyaluronic acid-collagen hydrogels promote cell spreading, fiber remodeling, and focal adhesion formation in 3D cell culture

自愈水凝胶 细胞外基质 材料科学 粘弹性 三维细胞培养 应力松弛 粘附 焦点粘着 细胞粘附 透明质酸 生物物理学 脚手架 细胞 组织工程 化学 纳米技术 生物医学工程 高分子化学 复合材料 解剖 生物化学 生物 蠕动 医学
作者
Junzhe Lou,Ryan S. Stowers,Sungmin Nam,Yan Xia,Ovijit Chaudhuri
出处
期刊:Biomaterials [Elsevier BV]
卷期号:154: 213-222 被引量:410
标识
DOI:10.1016/j.biomaterials.2017.11.004
摘要

The physical and architectural cues of the extracellular matrix (ECM) play a critical role in regulating important cellular functions such as spreading, migration, proliferation, and differentiation. Natural ECM is a complex viscoelastic scaffold composed of various distinct components that are often organized into a fibrillar microstructure. Hydrogels are frequently used as synthetic ECMs for 3D cell culture, but are typically elastic, due to covalent crosslinking, and non-fibrillar. Recent work has revealed the importance of stress relaxation in viscoelastic hydrogels in regulating biological processes such as spreading and differentiation, but these studies all utilize synthetic ECM hydrogels that are non-fibrillar. Key mechanotransduction events, such as focal adhesion formation, have only been observed in fibrillar networks in 3D culture to date. Here we present an interpenetrating network (IPN) hydrogel system based on HA crosslinked with dynamic covalent bonds and collagen I that captures the viscoelasticity and fibrillarity of ECM in tissues. The IPN hydrogels exhibit two distinct processes in stress relaxation, one from collagen and the other from HA crosslinking dynamics. Stress relaxation in the IPN hydrogels can be tuned by modulating HA crosslinker affinity, molecular weight of the HA, or HA concentration. Faster relaxation in the IPN hydrogels promotes cell spreading, fiber remodeling, and focal adhesion (FA) formation - behaviors often inhibited in other hydrogel-based materials in 3D culture. This study presents a new, broadly adaptable materials platform for mimicking key ECM features of viscoelasticity and fibrillarity in hydrogels for 3D cell culture and sheds light on how these mechanical and structural cues regulate cell behavior.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
追寻冰淇淋应助wpz采纳,获得50
刚刚
源圈圈完成签到 ,获得积分10
刚刚
1秒前
1秒前
1秒前
1秒前
冷傲天川发布了新的文献求助10
2秒前
付品聪发布了新的文献求助10
2秒前
4秒前
sciiiiii发布了新的文献求助10
4秒前
乒坛巨人发布了新的文献求助10
4秒前
阑悦关注了科研通微信公众号
4秒前
英俊的铭应助明天会更好采纳,获得10
4秒前
5秒前
5秒前
6秒前
Newt完成签到,获得积分10
6秒前
aaa发布了新的文献求助10
8秒前
9秒前
10秒前
反杀闰土的猹完成签到,获得积分10
10秒前
10秒前
Xieyusen发布了新的文献求助10
10秒前
芒芒发布了新的文献求助30
11秒前
开放惜寒发布了新的文献求助10
11秒前
13秒前
荒年完成签到,获得积分10
14秒前
aaa完成签到,获得积分20
15秒前
慕青应助冷傲天川采纳,获得10
15秒前
15秒前
16秒前
好运接收集成器完成签到,获得积分20
17秒前
18秒前
sciDoge完成签到,获得积分10
18秒前
19秒前
Bryan应助林狗采纳,获得10
19秒前
深情安青应助乒坛巨人采纳,获得10
20秒前
机智的邪欢完成签到,获得积分10
20秒前
大观天下完成签到,获得积分10
21秒前
anna发布了新的文献求助10
21秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Cognitive Neuroscience: The Biology of the Mind 1000
Technical Brochure TB 814: LPIT applications in HV gas insulated switchgear 1000
Immigrant Incorporation in East Asian Democracies 600
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
A Preliminary Study on Correlation Between Independent Components of Facial Thermal Images and Subjective Assessment of Chronic Stress 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3966989
求助须知:如何正确求助?哪些是违规求助? 3512429
关于积分的说明 11163148
捐赠科研通 3247241
什么是DOI,文献DOI怎么找? 1793778
邀请新用户注册赠送积分活动 874603
科研通“疑难数据库(出版商)”最低求助积分说明 804432