Influence of the properties of granitic rocks on their bioreceptivity to subaerial phototrophic biofilms

As any stone substrate is susceptible to biological colonisation, the choice of lithotype used for construction is a key strategy for preventing biodeterioration. For this purpose, a comprehensive evaluation of the primary bioreceptivity to phototrophic biofilms of eleven varieties of granitic rocks, commonly used as building material, was carried out. Blocks were inoculated with a multi-species phototrophic culture and subjected to standardised growth conditions for three months. Biofilm formation was assessed by chlorophyll (chl) fluorescence, colour measurements and extracellular polymeric substances (EPS) quantification. Relationships between the biofilm growth indicators and the properties of the different rocks studied were then analysed. Results showed that the bioreceptivity of the granites is more strongly affected by the physical characteristics of the stones than by their chemical and mineralogical properties, possibly because of the similar composition of the rocks studied. Growth of phototrophic biofilms was enhanced by high open porosity, capillary water content and surface roughness, and the bioreceptivity of weathered granites was higher than that of sound granites. The results obtained can therefore help in the selection of appropriate lithotypes for building purposes. The amounts of EPS produced by subaerial biofilms primarily depended on the requirements and/or characteristics of the biofilm-forming microorganisms, rather than on the bioreceptivity of the substratum, and microorganisms produce the amounts of EPS required at the initial stage of establishment on the stone surface, independently of the subsequent biomass development. These findings are especially important from the point of view of biodeterioration, in which the EPS matrix plays a central role.