亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Urinary afamin levels are associated with the progression of diabetic nephropathy

医学 糖尿病肾病 肌酐 肾功能 泌尿系统 糖尿病 肾脏疾病 尿 内科学 泌尿科 内分泌学 肾病 蛋白尿
作者
Yasushi Kaburagi,Eri Takahashi,Hiroshi Kajio,Satoshi Yamashita,Ritsuko Yamamoto‐Honda,Tomoko Shiga,Akinori Okumura,Atsushi Goto,Yuka Fukazawa,Norio Seki,Kazuyuki Tobe,Michihiro Matsumoto,Mitsuhiko Noda,Hiroyuki Unoki-Kubota
出处
期刊:Diabetes Research and Clinical Practice [Elsevier BV]
卷期号:147: 37-46 被引量:16
标识
DOI:10.1016/j.diabres.2018.02.034
摘要

Aims In this study, we applied quantitative proteomic analysis to identify urinary proteins associated with diabetic nephropathy (DN). Methods Two-dimensional image-converted analysis of liquid chromatography and mass spectrometry detected the proteins differentially excreted between normoalbuminuric and macroalbuminuric patients with type 2 diabetes mellitus (T2DM) (n = 6 each). Urinary levels of excreted proteins were measured by multiple reaction monitoring (MRM) analysis using an independent sample set (n = 77). Urinary afamin levels were measured by ELISA in T2DM and DN patients enrolled in this cohort study (n = 203). Results One-hundred-four proteins displayed significant alterations in excretion. Nine of these candidates were validated by MRM analysis. Among them, the levels of afamin, CD44 antigen, and lysosome-associated membrane glycoprotein 2, which have not previously been implicated in DN, were significantly associated with both the urinary albumin to creatinine ratio (ACR) and eGFR. We further measured afamin levels in urine collected from T2DM patients who did not yet have significant kidney disease (ACR < 300 mg/g or eGFR change rate ≤ 3.3%/year). The urinary afamin to creatinine ratio (Afa/Cre) was significantly higher in patients who progressed to a more severe DN stage or had early renal decline than in patients who did not. Conclusions Afa/Cre was significantly increased in T2DM patients who subsequently developed DN. Afa/Cre may be useful to predict patients with T2DM at high risk of nephropathy before the development of macroalbuminuria or reduced kidney function, although further validation studies in a larger population are needed.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
李健的粉丝团团长应助lzzz采纳,获得10
3秒前
6秒前
刘紫媛发布了新的文献求助10
11秒前
Raul完成签到 ,获得积分10
54秒前
lql完成签到 ,获得积分10
1分钟前
可爱的函函应助鲅鱼圈采纳,获得10
1分钟前
1分钟前
lzzz发布了新的文献求助10
1分钟前
英喆完成签到 ,获得积分10
1分钟前
我是老大应助畅快的毛衣采纳,获得10
2分钟前
2分钟前
2分钟前
鲅鱼圈发布了新的文献求助10
2分钟前
2分钟前
Leofar完成签到 ,获得积分10
2分钟前
2分钟前
鲅鱼圈完成签到,获得积分10
2分钟前
h0jian09完成签到,获得积分10
2分钟前
BaooooooMao完成签到,获得积分10
3分钟前
愉快的犀牛完成签到 ,获得积分10
3分钟前
Sunny完成签到,获得积分10
3分钟前
yujie完成签到 ,获得积分10
3分钟前
赘婿应助科研通管家采纳,获得10
4分钟前
4分钟前
西柚柠檬完成签到 ,获得积分10
4分钟前
心系天下完成签到 ,获得积分10
4分钟前
Alex-Song完成签到 ,获得积分0
4分钟前
不秃燃的小老弟完成签到 ,获得积分10
5分钟前
6分钟前
Owen应助科研通管家采纳,获得10
6分钟前
年年有余完成签到,获得积分10
6分钟前
胖小羊完成签到 ,获得积分10
7分钟前
8分钟前
领导范儿应助科研通管家采纳,获得10
8分钟前
8分钟前
juan完成签到 ,获得积分10
9分钟前
学术小垃圾完成签到,获得积分10
9分钟前
叁月二完成签到 ,获得积分10
9分钟前
9分钟前
科研通AI2S应助科研通管家采纳,获得10
10分钟前
高分求助中
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3990447
求助须知:如何正确求助?哪些是违规求助? 3532166
关于积分的说明 11256513
捐赠科研通 3271046
什么是DOI,文献DOI怎么找? 1805207
邀请新用户注册赠送积分活动 882302
科研通“疑难数据库(出版商)”最低求助积分说明 809234