RPO
生物
突变体
大肠杆菌
互补
饥饿
西格玛因子
肠杆菌科
生物化学
基因
基因表达
发起人
内分泌学
RNA聚合酶
作者
David M. Rockabrand,Kevin Livers,Tess Austin,Robyn Kaiser,Debra Bridges Jensen,Richard R. Burgess,Paul H. Blum
标识
DOI:10.1128/jb.180.4.846-854.1998
摘要
ABSTRACT DnaK is essential for starvation-induced resistance to heat, oxidation, and reductive division in Escherichia coli . Studies reported here indicate that DnaK is also required for starvation-induced osmotolerance, catalase activity, and the production of the RpoS-controlled Dps (PexB) protein. Because these dnaK mutant phenotypes closely resemble those of rpoS (ς 38 ) mutants, the relationship between DnaK and RpoS was evaluated directly during growth and starvation at 30°C in strains with genetically altered DnaK content. A starvation-specific effect of DnaK on RpoS abundance was observed. During carbon starvation, DnaK deficiency reduced RpoS levels threefold, while DnaK excess increased RpoS levels nearly twofold. Complementation of the dnaK mutation restored starvation-induced RpoS levels to normal. RpoS deficiency had no effect on the cellular concentration of DnaK, revealing an epistatic relationship between DnaK and RpoS. Protein half-life studies conducted at the onset of starvation indicate that DnaK deficiency significantly destabilized RpoS. RpoH (ς 32 ) suppressors of the dnaK mutant with restored levels of RpoS and dnaK rpoS double mutants were used to show that DnaK plays both an independent and an RpoS-dependent role in starvation-induced thermotolerance. The results suggest that DnaK coordinates sigma factor levels in glucose-starved E. coli.
科研通智能强力驱动
Strongly Powered by AbleSci AI