核受体辅活化子1
交易激励
核受体
核受体辅活化子3
毛皮-1
核受体辅活化子2
辅活化剂
细胞生物学
生物
雌激素受体
细胞生长
受体
雌激素相关受体γ
核受体辅阻遏物1
转录因子
原癌基因酪氨酸蛋白激酶Src
信号转导
生物化学
基因
遗传学
癌症
乳腺癌
作者
Hitoshi Tai,Naoki Kubota,Shigeaki Kato
标识
DOI:10.1006/bbrc.1999.1954
摘要
Steroid hormones regulate cell growth and function through the transcriptional control of target genes by their cognate nuclear receptors. These receptors bind to ligands and associate with transcriptional cofactors to stimulate transcription. SRC-1, one of the nuclear receptor coactivators, is known to interact with nuclear receptors and enhance transactivation function in a ligand-dependent manner. In this study, to assess the function of SRC-1 in cell growth regulated by nuclear receptor ligands, we established a stable transformant cell line overexpressing human SRC-1 and studied the action of 17beta-estradiol (E(2)) on cell growth as well as the expression of E(2)-responsive genes in MCF-7 cells. We found that SRC-1 overexpression potentiates cell growth stimulated by E(2) in accordance with enhancement of transcriptional activation of exogenous and endogenous E(2)-responsive genes. These findings clearly indicate the importance of nuclear receptor coactivators for the activities of steroid/lipophilic vitamins in cell growth and gene expression.
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