排尿
寡核苷酸
不稳定性
化学
糖苷键
核苷酸
嘌呤
色谱法
核酸
组合化学
有机化学
立体化学
生物化学
DNA
酶
基因
作者
Achim H. Krotz,Douglas L. Cole,Vasulinga T. Ravikumar
标识
DOI:10.1081/ncn-120019499
摘要
The removal of 4,4'-dimethoxytrityl (DMTr) groups from oligonucleotides at low pH and the acid lability of the glycosidic linkage of purine nucleotides constitute an inherent conflict in preparative oligonucleotide chemistry. The use of a mildly acidic NaOAc buffer (10 mM, pH 3.0-3.2) allows adjustment of the pH in a range where the progress of the DMTr removal reaction can be monitored conveniently by HPLC and the optimum reaction time can be calculated. As a result, oligonucleotides with minimum depurination are obtained.
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