免疫分析
肝细胞癌
微球
单克隆抗体
多克隆抗体
抗体
化学
生物
内科学
医学
免疫学
化学工程
工程类
作者
Na Ji,Yun Hee Kang,Mi-Young Park,Chung Il Lee,Mi Kyung Kim,Dae Ghon Kim,Jong Wan Kim,Eun Young Song
标识
DOI:10.1515/cclm.2011.008
摘要
Serum cystatin B (CSTB) concentrations have been reported to be increased in patients with hepatocellular carcinoma compared to concentrations seen in normal subjects. In this study, we developed a "fluorescent microsphere immunoassay" (FMI) capable of specifically detecting CSTB in serum.The FMI used a microparticle conjugated polyclonal antibody to CSTB and biotinylated monoclonal antibody as capture protein and probe protein, respectively. The results were obtained using the Bio-Plex(200) system.The dose-response relationship between CSTB and fluorescent intensity showed linearity in the range 0-1000 pg/mL and 7 pg/mL, sensitivity lower than 11.2 pg/mL. This result revealed that the FMI system was more sensitive than enzyme-linked immunoassay (ELISA). Additionally, the FMI system used smaller sample volumes compared to ELISA.We measured CSTB with both the FMI and an ELISA procedure and compared the two methods. The CSTB concentrations in serum specimens as measured with the FMI assay system were similar to those measured with ELISA. Thus, the new FMI using the Bio-Plex system may be useful for detection of CSTB in human serum.
科研通智能强力驱动
Strongly Powered by AbleSci AI