Paper chromatographic resolution of aliphatic guanidino amines, diamines and polyamines

1,3-Diaminopropane has been detected, together with spermidine, in cucumber seeds. Quantitative estimation of the two metabolites in cucumber seeds and germinating seedlings (up to 21 days old) showed that the concentrations of both metabolites increased, then decreased with age. A metabolic sequence linking spermidine, 1,3-diaminopropane and the non-protein amino acid β-pyrazol-1-yl alanine was suggested.