细胞生物学
细胞周期
DNA损伤
定量蛋白质组学
细胞周期蛋白依赖激酶6
细胞周期检查点
蛋白质组学
信号转导
生物
细胞生长
细胞迁移
细胞
细胞周期蛋白
化学
DNA
生物化学
基因
作者
Zhe Qin,Haiyan Hou,Feng Fu,Jun Wu,Bin Han,Jing Wang,Liwen Zhang,Jin Cao,Xiaohan Jin,Shixiang Cheng,Zhen Yang,Min Zhang,Xiaoxia Lan,Ting Yao,Qulong Dong,Siyu Wu,Jingjing Zhang,Zhongwei Xu,Yuming Li,Yaqiong Chen
标识
DOI:10.1016/j.reprotox.2017.08.014
摘要
Long-term exposure to fine particulate matter (PM2.5) may cause adverse pregnancy outcomes but the mechanisms are not clear. Our research confirms that PM2.5 induced DNA damage, and inhibited cell proliferation in HTR-8/SVneo cells, presenting in a dose- and time-dependent manners. Using quantitative proteomics, the 182 and 486 differentially expressed proteins in cells treated with 120 μg ml−1 PM2.5 for 24 and 48 h were involved in many critical biological processes, including of cell proliferation, response to DNA damage, regulation of small GTPase mediated signal transduction, and etc. Further validation indicated that PM2.5 blocked the cell cycle at the G2/M phase through activation of the ATR-Cyclin E1/Cdk6 pathway, and it reduced the migration and invasion by upregulating TIMP1 and TIMP2 expression and downregulating Collagen I expression. Our findings were consistent with the observed effects of PM2.5 on cell cycle arrest and inhibition of migration and invasion in human extravillous trophoblast.
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