薄层荧光显微镜
荧光
荧光寿命成像显微镜
显微镜
光激活定位显微镜
生物物理学
化学
荧光显微镜
分辨率(逻辑)
纳米技术
图像分辨率
活体细胞成像
时间分辨率
穿透深度
双光子激发显微术
光学
显微镜
超分辨显微术
材料科学
生物
计算机科学
物理
人工智能
细胞
生物化学
作者
Xiaohong Peng,Xiaoshuai Huang,Ke Du,Huisheng Liu,Liangyi Chen
出处
期刊:Biochemical Society Transactions
[Portland Press]
日期:2019-12-12
卷期号:47 (6): 1635-1650
被引量:15
摘要
Taking advantage of high contrast and molecular specificity, fluorescence microscopy has played a critical role in the visualization of subcellular structures and function, enabling unprecedented exploration from cell biology to neuroscience in living animals. To record and quantitatively analyse complex and dynamic biological processes in real time, fluorescence microscopes must be capable of rapid, targeted access deep within samples at high spatial resolutions, using techniques including super-resolution fluorescence microscopy, light sheet fluorescence microscopy, and multiple photon microscopy. In recent years, tremendous breakthroughs have improved the performance of these fluorescence microscopies in spatial resolution, imaging speed, and penetration. Here, we will review recent advancements of these microscopies in terms of the trade-off among spatial resolution, sampling speed and penetration depth and provide a view of their possible applications.
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