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Probiotic potential of Saccharomyces cerevisiae and Kluyveromyces marxianus isolated from West African spontaneously fermented cereal and milk products

克鲁维酵母 益生菌 生物 酿酒酵母 酵母 发酵 碳酸钙-2 食品科学 拉伤 克鲁维酵母 微生物学 开菲尔 生物化学 细菌 细胞 乳酸 遗传学 解剖
作者
Grace Adzo Motey,Pernille Johansen,James Owusu‐Kwarteng,Linda Aurelia Ofori,Kwasi Obiri‐Danso,Henrik Siegumfeldt,Nadja Larsen,Lene Jespersen
出处
期刊:Yeast [Wiley]
卷期号:37 (9-10): 403-412 被引量:20
标识
DOI:10.1002/yea.3513
摘要

Abstract The yeast species Saccharomyces cerevisiae and Kluyveromyces marxianus are associated with fermentation of West African indigenous foods. The aim of this study was to characterize potential probiotic properties of S. cerevisiae and K. marxianus isolates from the West African milk products lait caillé and nunu and a cereal‐based product mawè. The strains (14 in total) were identified by 26S rRNA gene sequencing and characterized for survival at gastrointestinal stress (bile salts and low pH) and adhesion to Caco‐2 intestinal epithelial cells. Selected yeast isolates were tested for their effect on the transepithelial electrical resistance (TEER), using the intestinal epithelial cell line Caco‐2 and for maintenance of intracellular pH (pH i ) during perfusion with gastrointestinal pH (3.5 and 6.5). All tested yeasts were able to grow in bile salts in a strain‐dependent manner, exhibiting a maximum specific growth rate (μ max ) of 0.58–1.50 h −1 . At pH 2.5, slow growth was observed for the isolates from mawè (μ max of 0.06–0.80 h −1 ), whereas growth of yeasts from other sources was mostly inhibited. Yeast adhesion to Caco‐2 cells was strain specific and varied between 8.0% and 36.2%. Selected strains of S. cerevisiae and K. marxianus were able to maintain the pH i homeostasis at gastrointestinal pH and to increase TEER across the Caco‐2 monolayers, indicating their potential to improve intestinal barrier functions. Based on overall results, strains of K. marxianus and S. cerevisiae from mawè exhibited the highest probiotic potential and might be recommended for further development as starter cultures in West African fermented products.
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