Ropivacaine-induced seizures evoked pain sensitization in rats: Participation of 5-HT/5-HT3R

医学 麻醉 罗哌卡因 敏化 局部麻醉剂 麻醉剂 伤害 伤害感受器 内科学 受体 免疫学
作者
Chen-Long Yang,Jun-Jie Jing,Si-Yin Fu,Yu-Ling Zhong,Xiu-Zhu Su,Zhong-Mou Shi,Xiaozhi Wu,Fei Yang,Guozhong Chen
出处
期刊:Neurotoxicology [Elsevier BV]
卷期号:93: 173-185 被引量:2
标识
DOI:10.1016/j.neuro.2022.10.001
摘要

Due to the increasing use of local anesthetic techniques in various healthcare settings, local anesthetic toxicity still occurs. Seizures are the most common symptom of local anesthetic toxicity. The relationship between local anesthetic-induced seizures and the sensation of pain has not been established till now. Here, we assessed the development of pain hypersensitivity after ropivacaine-induced seizures (RIS) and the influence of RIS on incision-induced postsurgical pain and formalin-induced acute inflammatory pain. In addition, the involvement of spinal 5-HT/5-HT3R in RIS-induced pain sensitization was investigated. According to a sequential exploratory experimental strategy, we first calculated the 50% seizure dosage of ropivacaine to be 42.66 mg/kg (95% confidence interval: 40.19–45.28 mg/kg). We showed that RIS induced significant bilateral mechanical pain hypersensitivity that lasted around 5 days, accompanied by an increase in spinal 5-HT. Moreover, RIS considerably protracted postsurgical pain and enhanced formalin-induced spontaneous flinching in the second phase. Depletion of spinal 5-HT with intrathecal injection of 5,7-dihydroxytryptamine (5,7-DHT) reduced RIS-induced pain hypersensitivity and prevented the prolonging of postsurgical pain following RIS. Likewise, blocking spinal 5-HT3R by intrathecal administration of ondansetron reversed RIS-induced pain hypersensitivity and attenuated the pronociception of RIS in the formalin test. Our findings revealed that acute RIS led to pain hypersensitivity and had pronociceptive effects on incision-induced postsurgical pain and formalin-induced acute inflammatory pain. Moreover, our data implied that RIS-induced pain sensitization depends on spinal 5-HT/5-HT3R signaling. Thus, targeting the descending serotonergic facilitation system should be an important element of the precise treatment for local anesthetic toxicity.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
苹果从菡发布了新的文献求助10
刚刚
fyl发布了新的文献求助30
刚刚
1秒前
bronny完成签到,获得积分10
1秒前
2秒前
吴糖完成签到,获得积分10
2秒前
2秒前
谦让沛槐完成签到,获得积分10
2秒前
谨慎秋珊发布了新的文献求助10
2秒前
舒适的尔容完成签到,获得积分10
3秒前
3秒前
ljxx发布了新的文献求助10
3秒前
充电宝应助张津浩采纳,获得10
3秒前
美姿完成签到 ,获得积分10
3秒前
小马甲应助hinini采纳,获得10
3秒前
要开心完成签到 ,获得积分10
4秒前
执着的灯泡完成签到,获得积分10
5秒前
芒果布丁发布了新的文献求助10
5秒前
master发布了新的文献求助10
5秒前
6秒前
元来完成签到,获得积分10
6秒前
6秒前
akber123完成签到,获得积分10
7秒前
乐乐应助谦让沛槐采纳,获得10
7秒前
卫川影发布了新的文献求助10
7秒前
8秒前
z_king_d_23发布了新的文献求助10
8秒前
hp571发布了新的文献求助10
8秒前
灵巧的翠桃完成签到,获得积分10
8秒前
许安完成签到,获得积分10
9秒前
10秒前
10秒前
10秒前
soul发布了新的文献求助10
11秒前
JamesPei应助yinghan1212采纳,获得10
11秒前
百事可乐应助无语啦采纳,获得10
12秒前
等DENG完成签到,获得积分10
12秒前
zly完成签到,获得积分10
12秒前
abcdulla777完成签到,获得积分20
12秒前
Lmj发布了新的文献求助10
13秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Cognitive Neuroscience: The Biology of the Mind 1000
Technical Brochure TB 814: LPIT applications in HV gas insulated switchgear 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Picture Books with Same-sex Parented Families: Unintentional Censorship 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3969335
求助须知:如何正确求助?哪些是违规求助? 3514162
关于积分的说明 11172430
捐赠科研通 3249456
什么是DOI,文献DOI怎么找? 1794853
邀请新用户注册赠送积分活动 875437
科研通“疑难数据库(出版商)”最低求助积分说明 804809