Bioceramic and polycationic biopolymer nanocomposite scaffolds for improved wound self-healing and anti-inflammatory properties: an in vitro study

The development of wound healing scaffolds with high porosity, rapid healing properties, and anti-inflammatory functionality is vital in the chronic wound healing stage for the production of extracellular matrices of injured tissues. The 45S5 bioactive glass (BG) possesses good biocompatibility and provides a potential bonding resource for fibroblast cell proliferation, growth factor synthesis, and granulated tissue formation. Chitosan, a natural polymer, promotes tissue regeneration and has anti-microbial properties. BG and chitosan scaffolds were prepared by the freeze-drying (lyophilization) method. The chitosan scaffold is a semi-crystalline polymer with a random crystal structure because it contains more hydroxyl groups. Chitosan alone shows a sheet-like morphology with a porous microstructure (1.7475 nm). BG particulates were well decorated over the surface of the chitosan scaffold with a homogeneous dispersion. Cell viability was observed for L929 cells on the chitosan-BG scaffolds. Confocal images vividly depict the interaction of the L929 cells with the scaffold without causing any damage to the cell membrane. In vitro scratch assay shows the best wound healing activity (complete wound closure) for the BG-chitosan nanocomposite scaffolds at 18 h. The chitosan-BG scaffolds were combined with anti-inflammatory drugs and induced inflammatory genes at an inhibition rate of COX of (36, 28, and 30%), LOX of (20, 13, and 14%), and NO of (48, 38, and 39%) for chitosan, chitosan-BG, and chitosan-BG (Na-free) at 100 μL addition. The in vitro bioactivities proved that the chitosan-BG scaffolds could enable better cell formation, and exhibited improved biocompatibility, and anti-inflammatory and wound healing properties.