极性(国际关系)
内质网
细胞生物学
荧光
生物物理学
荧光显微镜
共焦
细胞内
脂质过氧化
化学
共焦显微镜
电池极性
扫描电镜
生物
细胞
生物化学
物理
光学
激光器
氧化应激
受激发射
作者
Ming‐Feng Li,Yuncong Chen,Weijiang He,Zijian Guo
标识
DOI:10.1002/chem.202401285
摘要
As a new form of regulated cell death, ferroptosis is closely related to various diseases. Tracing ferroptosis related biological behavior is helpful to better understand this process and its related biology. Considering that ferroptosis is featured with remarkable lipid peroxidation which can easily change the membranes' compositions and structures, it is potential to detect intracellular environmental changes for direct assessment of ferroptosis. In view of the close relationship between endoplasmic reticulum (ER) and ferroptosis, we designed an ER-targeted and polarity-sensitive fluorescent probe SBD-CH, which has superior photostability and can respond to polarity with high selectivity without the affection of viscosity. SBD-CH can monitor the trend of ER polarity during ferroptosis by confocal laser scanning microscopy (CLSM), and analyze the distribution of polarity in ferroptosis by fluorescence lifetime imaging microscopy (FLIM). During Erastin induced ferroptosis, the polarity of ER in HT-1080 cells increased and the polarity distribution in ER was more dispersed. Our work provides an effective strategy for evaluating the process of ferroptosis by monitoring the changes of ER polarity.
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