Fluorescent Probe for Imaging Hydrogen Polysulfide Dynamics of Macrophage Inflammatory Regulation in Bone Homeostasis

Herein, a fluorescent probe BFO-HPS was developed for imaging the H2Sn dynamics of the macrophage inflammatory regulation in bone homeostasis. The probe indicated advantages including large response scale, rapid response, steady optical performance in various pH conditions, high selectivity, and good selectivity. BFO-HPS showed a dose-dependent fluorescence response at 570 nm for the H2Sn concentration with the linear range from 0.1 to 100 µM, limit of detection of 0.11 µM, and limit of quantitation of 0.10 µM. Based on the low cyto-toxicity, BFO-HPS provided the intracellular monitoring of the exogenous H2Sn level in uninduced RAW264.7 cells. In the receptor activator of nuclear factor-κB ligand (RANKL)-induced and RANKL-induced inhibited groups, BFO-HPS achieved the monitoring of the H2Sn dynamics to indicate the macrophage-involved bone homeostasis. This work was informative for further investigations on macrophage inflammatory regulation in bone homeostasis.