Co-methylation analyses identify CpGs associated with lipid traits in Chinese discordant monozygotic twins

生物 DNA甲基化 混淆 甲基化 单卵双胞胎 遗传力缺失问题 遗传学 高密度脂蛋白 双胞胎研究 内科学 基因 遗传关联 内分泌学 遗传力 胆固醇 肿瘤科 单核苷酸多态性 基因表达 医学 基因型
作者
Zhaoying Li,Weijing Wang,Weilong Li,Haiping Duan,Chunsheng Xu,Xiaocao Tian,Na Feng,Dongfeng Zhang
出处
期刊:Human Molecular Genetics [Oxford University Press]
标识
DOI:10.1093/hmg/ddad207
摘要

To control genetic background and early life milieu in genome-wide DNA methylation analysis for blood lipids, we recruited Chinese discordant monozygotic twins to explore the relationships between DNA methylations and total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), and triglycerides (TG). 132 monozygotic (MZ) twins were included with discordant lipid levels and completed data. A linear mixed model was conducted in Epigenome-wide association study (EWAS). Generalized estimating equation model was for gene expression analysis. We conducted Weighted correlation network analysis (WGCNA) to build co-methylated interconnected network. Additional Qingdao citizens were recruited for validation. Inference about Causation through Examination of Familial Confounding (ICE FALCON) was used to infer the possible direction of these relationships. A total of 476 top CpGs reached suggestively significant level (P < 10-4), of which, 192 CpGs were significantly associated with TG (FDR < 0.05). They were used to build interconnected network and highlight crucial genes from WGCNA. Finally, four CpGs in GATA4 were validated as risk factors for TC; six CpGs at ITFG2-AS1 were negatively associated with TG; two CpGs in PLXND1 played protective roles in HDL-C. ICE FALCON indicated abnormal TC was regarded as the consequence of DNA methylation in CpGs at GATA4, rather than vice versa. Four CpGs in ITFG2-AS1 were both causes and consequences of modified TG levels. Our results indicated that DNA methylation levels of 12 CpGs in GATA4, ITFG2-AS1, and PLXND1 were relevant to TC, TG, and HDL-C, respectively, which might provide new epigenetic insights into potential clinical treatment of dyslipidemia.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
2秒前
NiNi完成签到,获得积分10
3秒前
Ther完成签到,获得积分20
4秒前
傲娇白安完成签到,获得积分10
5秒前
5秒前
甜蜜的荟完成签到,获得积分20
6秒前
婷儿完成签到,获得积分10
6秒前
牛牛发布了新的文献求助10
6秒前
Hoper完成签到,获得积分10
6秒前
张曰淼完成签到,获得积分10
7秒前
共渡完成签到,获得积分10
9秒前
凉白开完成签到 ,获得积分10
10秒前
跳跃的太君完成签到,获得积分10
11秒前
小猪发布了新的文献求助10
11秒前
独特问夏完成签到,获得积分10
12秒前
12秒前
13秒前
魔幻蓉完成签到,获得积分10
13秒前
杠赛来完成签到,获得积分10
13秒前
ccy完成签到 ,获得积分10
14秒前
Ch185完成签到,获得积分10
15秒前
欣喜的复天完成签到,获得积分10
17秒前
摸鱼校尉完成签到,获得积分0
17秒前
双儿完成签到,获得积分10
17秒前
顺利毕业完成签到 ,获得积分10
17秒前
儒雅的焦完成签到 ,获得积分10
17秒前
小何完成签到 ,获得积分10
17秒前
19秒前
20秒前
小曾应助张绪帆采纳,获得10
20秒前
麻麻薯完成签到 ,获得积分10
21秒前
21秒前
Smes完成签到,获得积分10
21秒前
王金豪发布了新的文献求助10
22秒前
勤劳冰烟完成签到,获得积分10
22秒前
顺顺尼完成签到 ,获得积分10
23秒前
24秒前
阿曾发布了新的文献求助10
25秒前
25秒前
鹤昀完成签到 ,获得积分10
26秒前
高分求助中
【提示信息,请勿应助】关于scihub 10000
Les Mantodea de Guyane: Insecta, Polyneoptera [The Mantids of French Guiana] 3000
徐淮辽南地区新元古代叠层石及生物地层 3000
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
Handbook of Industrial Diamonds.Vol2 1100
Global Eyelash Assessment scale (GEA) 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 550
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4038368
求助须知:如何正确求助?哪些是违规求助? 3576068
关于积分的说明 11374313
捐赠科研通 3305780
什么是DOI,文献DOI怎么找? 1819322
邀请新用户注册赠送积分活动 892672
科研通“疑难数据库(出版商)”最低求助积分说明 815029