生物正交化学
四嗪
荧光团
多路复用
化学
荧光
组合化学
生物结合
纳米技术
点击化学
生物物理学
材料科学
生物
生物信息学
有机化学
物理
量子力学
作者
Yingqiao Deng,Tianruo Shen,Xinyu Yu,Jie Li,Peixuan Zou,Qiyong Gong,Yongxiang Zheng,Hongbao Sun,Xiaogang Liu,Haoxing Wu
标识
DOI:10.1002/anie.202319853
摘要
Developing fluorogenic probes for simultaneous live cell labeling of multiple targets is crucial for understanding complex cellular events. The emerging [4+1] cycloaddition between tetrazine and isonitriles holds promise as a bioorthogonal tool, yet existing tetrazine probes lack reactivity and fluorogenicity. Here, we present the development of a series of tetrazine-functionalized bioorthogonal probes. By incorporating pyrazole adducts into the fluorophore scaffolds, the post-reacted probes displayed remarkable fluorescence turn-on ratios, up to 3184-fold. Moreover, these modifications are generalizable to various fluorophores, enabling a broad emission range from 473 to 659 nm. Quantum chemical calculations further elucidate the turn-on mechanisms. These probes enable the simultaneous labeling of multiple targets in live cells, without the need for a washing step. Consequently, our findings pave the way for advanced multiplex imaging and detection techniques for cellular studies.
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