氯氟氰菊酯
生物
RNA干扰
中肠
G蛋白偶联受体
基因
戒毒(替代医学)
下调和上调
受体
基因表达
幼虫
分子生物学
遗传学
核糖核酸
杀虫剂
植物
生态学
医学
病理
替代医学
作者
Yuxi Liu,Chao Hu,Yuting Li,Ping Gao,Xue Yang
标识
DOI:10.1021/acs.jafc.3c06522
摘要
While previous studies have reported G protein-coupled receptor (GPCR)-mediated insecticide resistance in various arthropods, the understanding of GPCR-associated resistance mechanisms in Cydia pomonella remains limited. In this study, a total of 95 CpGPCR genes categorized into four families were identified in C. pomonella. Results revealed high expression levels of the majority of the CpGPCRs during the first larval stage and in the head of C. pomonella. Exposure to lambda-cyhalothrin significantly increased the expression of 15 CpGPCRs, including CpGPCR70, which is highly expressed in all larval stages and shows the highest expression in the midgut. RNA interference (RNAi) demonstrated that downregulation of CpGPCR70 leads to reduced expression of key resistance-related genes and a decreased tolerance of larvae to lambda-cyhalothrin. These findings indicate that CpGPCR70 plays a crucial role in regulating the expression of detoxifying genes involved in lambda-cyhalothrin resistance, offering valuable insights for the development of more effective pest control strategies.
科研通智能强力驱动
Strongly Powered by AbleSci AI