欧洲越橘
细胞凋亡
体内
花青素
黑色素瘤
达卡巴嗪
药理学
PI3K/AKT/mTOR通路
myrtillus越桔
蛋白激酶B
化学
癌症研究
MCF-7型
细胞生长
活性氧
医学
生物
癌症
生物化学
癌细胞
内科学
食品科学
生物技术
植物
人体乳房
作者
Xuehong Yu,Yuhua Yue,Haizhu Shi,Kaiyan Xu,Chungu Zhang,Yu Wan,Shun Feng
标识
DOI:10.1080/01635581.2022.2157450
摘要
Anthocyanins have been reported to have potential as dietary or pharmaceutical supplements in the application of cancer prevention and adjunctive treatment. However, there are few studies on the effect of anthocyanins on melanoma, which have only been performed in cell lines. The objective of this work was to investigate the anticancer effects and mechanisms of bilberry anthocyanin extract (BAE) on melanoma In Vitro and In Vivo. Moreover, a primary study was done to investigate how BAE influenced C57BL/6 mice bearing subcutaneous B16-F10 tumors treated with dacarbazine (DTIC). BAE-induced apoptosis in B16-F10 cells was associated with activation of the mitochondrial pathway induced by increased reactive oxygen species. More, In Vivo anticancer activity studies indicated that BAE attenuated melanoma growth, as identified by hematoxylin-eosin staining, Ki-67, and TUNEL assays. Further western blot results revealed higher phospho-Akt expression with the combination of BAE and DTIC, indicating no suppression of the PI3K/AKT signaling pathway. In summary, this study demonstrated the anti-melanoma activity of BAE and investigated its mechanism. Notably, it should be careful to use products enriching BAE for those melanoma patients treated with DTIC.
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