[miR-29a-3p Targets Hepatoma-Derived Growth Factor to Inhibit the Proliferation and Promote the Apoptosis of E6-1 Cells].

To investigate the regulatory effect of miRNA-29a-3p (miR-29a-3p ) on hepatoma-derived growth factor (HDGF), and its influences on the proliferation and apoptosis of acute lymphoblastic leukemia (ALL) cell line E6-1.Thirty-five patients with ALL treated in our hospital from January 2017 to January 2019 were selected as research objects, and 35 adults who underwent physical examination in the same period were selected as healthy control group. The miR-29a-3p overexpression vector, miR-29a-3p inhibitory expression vector, and miR-29a-3p and HDGF co-overexpression vector were transfected into E6-1 cells. The expression levels of miR-29a-3p and HDGF mRNA were detected by RT-qPCR. The expression of protein was detected by Western blot. The targeting relationship between miR-29a-3p and HDGF was detected by dual luciferase reporter assay. The cell proliferation was detected by CCK-8 method, while apoptosis detected by flow cytometry.Compared with healthy control group, HDGF was highly expressed in serum of patients with ALL and leukemia cells HuT 78, E6-1, CCRF-CEM, while miR-29a-3p was low expressed (P<0.05). After overexpression of miR-29a-3p , the expression levels of CyclinD1 and Bcl-2 in leukemia cells E6-1 were significantly reduced, while the expression levels of p21 and Bax were significantly increased (P<0.05). The activity of E6-1 cells was also significantly reduced, while the apoptosis rate of E6-1 cells was significantly increased (P<0.05). miR-29a-3p could target and regulate the expression of HDGF, while overexpression of HDGF reversed the inhibitory effect of miR-29a-3p overexpression on the proliferation and promotion effect on the apoptosis of leukemia cells E6-1.Overexpression of miR-29a-3p can inhibit the proliferation and promote the apoptosis of ALL cells E6-1, and its mechanism may be related to the regulation of HDGF expression.miR-29a-3p 靶向肝癌衍生生长因子抑制E6-1细胞增殖并促进其凋亡.探讨miRNA-29a-3p（miR-29a-3p ）对肝癌衍生生长因子（HDGF）的调控作用以及急性淋巴细胞白血病细胞系E6-1增殖、凋亡的影响。.选择2017年1月-2019年1月在本院就诊的急性淋巴细胞白血病患者35例，同期进行健康体检的成人35例为健康对照组。将miR-29a-3p 过表达载体、miR-29a-3p 抑制表达载体以及miR-29a-3p 和HDGF联合过表达载体转染至E6-1细胞中，用RT-qPCR检测miR-29a-3p 和HDGF mRNA的表达水平，Western blot检测蛋白表达，双荧光素酶报告实验检测miR-29a-3p 和HDGF的靶向关系，CCK-8法检测细胞增殖，流式细胞术检测细胞凋亡。.与健康对照组相比，急性淋巴细胞白血病患者血清及细胞HuT 78、E6-1、CCRF-CEM中HDGF高表达，miR-29a-3p 低表达（P<0.05）。过表达miR-29a-3p 后，白血病细胞E6-1中CyclinD1、Bcl-2表达水平显著降低，p21、Bax表达水平显著升高，E6-1细胞活性显著降低，凋亡率显著升高（均P<0.05）。miR-29a-3p 能靶向调控HDGF表达，过表达HDGF逆转了miR-29a-3p 过表达对白血病细胞E6-1的增殖抑制和凋亡促进作用。.miR-29a-3p 过表达可抑制急性淋巴细胞白血病细胞E6-1的增殖、促进其凋亡，其机制可能与调控HDGF的表达有关。.