Post‐transcriptional regulation of grain weight and shape by the RBP‐A‐J‐K complex in rice

生物 转录组 RNA结合蛋白 人口 功能(生物学) 基因 细胞生物学 核糖核酸 遗传学 基因表达 人口学 社会学
作者
Ren Ding,Hui Liu,Xuejun Sun,Fan Zhang,Ling Jiang,Ying Wang,Ning Jiang,Peiwen Yan,Jinhao Cui,Junhan Yang,Z.-K. Li,Pingli Lu,Xiaosheng Luo
出处
期刊:Journal of Integrative Plant Biology [Wiley]
标识
DOI:10.1111/jipb.13583
摘要

ABSTRACT RNA‐binding proteins (RBPs) are components of the post‐transcriptional regulatory system, but their regulatory effects on complex traits remain unknown. Using an integrated strategy involving map‐based cloning, functional characterizations, and transcriptomic and population genomic analyses, we revealed that RBP‐K ( LOC _ Os08g23120 ), RBP‐A ( LOC _ Os11g41890 ), and RBP‐J ( LOC _ Os10g33230 ) encode proteins that form an RBP‐A‐J‐K complex that negatively regulates rice yield‐related traits. Examinations of the RBP‐A‐J‐K complex indicated RBP‐K functions as a relatively non‐specific RBP chaperone that enables RBP‐A and RBP‐J to function normally. Additionally, RBP‐J most likely affects GA pathways, resulting in considerable increases in grain and panicle lengths, but decreases in grain width and thickness. In contrast, RBP‐A negatively regulates the expression of genes most likely involved in auxin‐regulated pathways controlling cell wall elongation and carbohydrate transport, with substantial effects on the rice grain filling process as well as grain length and weight. Evolutionarily, RBP‐K is relatively ancient and highly conserved, whereas RBP‐J and RBP‐A are more diverse. Thus, the RBP‐A‐J‐K complex may represent a typical functional model for many RBPs and protein complexes that function at transcriptional and post‐transcriptional levels in plants and animals for increased functional consistency, efficiency, and versatility as well as increased evolutionary potential. Our results clearly demonstrate the importance of RBP‐mediated post‐transcriptional regulation for the diversity of complex traits. Furthermore, rice grain yield and quality may be enhanced by introducing various complete or partial loss‐of‐function mutations to specific RBP genes using CRISPR/Cas9 technology and by exploiting desirable natural tri‐genic allelic combinations at the loci encoding the components of the RBP‐A‐J‐K complex through marker‐assisted selection. This article is protected by copyright. All rights reserved.
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