Isolation of Endophytic Bacteria from Leaves, Stems, or Roots

Following the discovery of endophytic bacteria in potato, sugarcane, and rice, isolation and characterization of the same has advanced over the years. Studies of these endophytes in plant tissues require techniques that would enable researchers to either locate them in the plant tissues or isolate them from the tissues for subsequent visualization. Over the years, endophytic diazotrophic bacteria have been isolated from root, culm, stem, and leaf tissues and characterized for their activities in different crop plants, including plant growth promoters and biological nitrogen fixation. Various methods and culture media have been used depending on the crop plant under study and available resources for the laboratory procedures.