Description of Veillonella absiana sp. nov., isolated from pig faeces

Two Gram-stain-negative cocci anaerobes were isolated from pig faeces and designated as strains YH-vei2232 T and YH-vei2233. Phylogenetic analysis using 16S rRNA gene sequences revealed that the isolates were most closely related to Veillonella rogosae KCTC 5967 T , with 97.0% similarity. Analysis of housekeeping gene sequences ( rpoB ) revealed the strain formed a sub-cluster within the genus Veillonella . The average nucleotide identities between the two isolates and the most closely related strains within genus Veillonella were <75.0%. The major fatty acids were Summed Feature 8 and C 16 : 1 ω9 c. The cell wall peptidoglycan contained meso -diaminopimelic acid. The major metabolic end products of isolates were propionic and acetic acids. The genomic DNA G+C contents of both strains were 40.1 mol%. The chemotaxonomic, phenotypic and phylogenetic properties of YH-vei2232 T (=KCTC 25748 T =NBRC 116427 T ) and YH-vei2233 (=KCTC 25749=NBRC 116428) suggest that they represent a novel species of the genus Veillonella , for which the name Veillonella absiana sp. nov. is proposed.