考马斯亮蓝
尿
排泄
泌尿系统
色谱法
化学
医学
内科学
生物化学
病理
染色
作者
James A. Lott,Volker Stephan,Kirkwood A. Pritchard
出处
期刊:Clinical Chemistry
[Oxford University Press]
日期:1983-11-01
卷期号:29 (11): 1946-1950
被引量:105
标识
DOI:10.1093/clinchem/29.11.1946
摘要
The Coomassie Brilliant Blue G-250 method for protein in urine has been evaluated for analytical accuracy and clinical applicability. Extremely simple to perform, the test exhibits good precision and sensitivity. The color developed per gram of protein is protein-type dependent, so no single protein standard is completely satisfactory. Color intensity is linearly related to concentration up to 1500 mg/L when used with a manual bichromatic method. Accuracy was clinically acceptable for patients with a variety of protein-losing diseases, and for patients having received renal transplants; however, the method underestimates urinary light-chain proteins. In athletes and premature neonates, we observed increased protein excretion during periods of stress. The upper reference limit for protein excretion in healthy adults is about 120 mg/24 h.
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