吞噬作用
内化
细胞生物学
调理素
巨噬细胞
免疫系统
先天免疫系统
受体
生物
免疫荧光
荧光显微镜
化学
抗体
微生物学
免疫学
荧光
生物化学
体外
物理
量子力学
作者
Christopher H. Choy,Roberto J. Botelho
出处
期刊:Methods in molecular biology
日期:2016-11-05
卷期号:: 43-53
被引量:2
标识
DOI:10.1007/978-1-4939-6581-6_4
摘要
Phagocytosis is the actin-driven internalization of solid particles, utilized by phagocytic immune cells to sequester potentially infectious microorganisms. Aided by the innate and adaptive immune system, the activation of various phagocytic receptors triggers a cascade of downstream signaling mediators that drive actin and plasma membrane remodeling. Modulation of these molecular players can lead to distinct changes in the capacity and rates of phagocytosis. Here, we present a fluorescence microscopy based technique to quantify phagocytosis using a macrophage-like cell line. We exemplify the technique through the phagocytosis of antibody-opsonized polystyrene beads. This method can be extended to other phagocytes and phagocytic particles.
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