生物
核酸
计算生物学
遗传学
荧光素酶
结核分枝杆菌
序列(生物学)
基因组
生物传感器
DNA
基因
生物化学
肺结核
转染
医学
病理
作者
Yihao Zhang,Long Qian,Weijia Wei,Yu Wang,Beining Wang,Pingping Lin,Wenchao Li,Luze Xu,Xiang Li,Dongming Liu,Sida Cheng,Jiaofeng Li,Yixuan Ye,Hang Li,Xiaohan Zhang,Yun Dong,Xuejin Zhao,Cuihua Liu,Haoqian Zhang,Qi Ouyang,Chunbo Lou
标识
DOI:10.1021/acssynbio.6b00215
摘要
We developed an in vitro DNA detection system using a pair of dCas9 proteins linked to split halves of luciferase. Luminescence was induced upon colocalization of the reporter pair to a ∼44 bp target sequence defined by sgRNAs. We used the system to detect Mycobacterium tuberculosis DNA with high specificity and sensitivity. The reprogrammability of dCas9 was further leveraged in an array design that accesses sequence information across the entire genome.
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