A novel near-infrared fluorescent probe for the dynamic monitoring of the concentration of glutathione in living cells

In this study, a water-soluble, near-infrared fluorescent probe (EQR-S) was designed for the measurement of the glutathione (GSH) concentration. Responses of different interfering substances to the developed probe were investigated, and the luminescence mechanism was examined by theoretical calculations. Results revealed that EQR-S can be applied for the rapid, sensitive determination of the GSH concentration with a detection limit of 69 nM. Based on the above advantages, EQR-S was successfully applied to investigate the fluctuation in the GSH concentration of living cells under high-temperature stress.