费斯特共振能量转移
荧光
化学
生物传感器
检出限
滚动圆复制
生物分子
信号(编程语言)
底漆(化妆品)
DNA
组合化学
生物物理学
纳米技术
色谱法
生物化学
物理
材料科学
光学
生物
DNA复制
计算机科学
有机化学
程序设计语言
作者
Hongkun Li,Qianqian Cai,Di Wu,Guifen Jie,Hong Zhou
标识
DOI:10.1016/j.aca.2022.340190
摘要
A novel fluorescence biosensor based on hyperbranched rolling circle amplication (HRCA) and multi-site strand displacement amplification (SDA) strategy was constructed to realize super-sensitive detection of miRNA-21. The target miRNA-21 was specifically used to trigger HRCA reaction and generated abundant DNA sequences with different lengths. These sequences could initiate the SDA reaction with hairpin HP1 and HP2 to form the fluorescence signal double-stranded bodies (FSDB), and produced the fluorescence resonance energy transfer (FRET) for target assay. The circle template, reverse primer, hairpin HP1 and HP2 were skillfully designed to much improve the assay selectivity. This FRET signal ratio-based strategy not only avoided the false positive signal produced by traditional detection methods, but also minimized the influence of system fluctuation. So the detection limit of the target could reach fM level. In addition, this method could also be applied to the detection of other miRNAs, proteins and biomolecules, and had great potential in biomedical research, environmental detection and clinical diagnostic applications.
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