芥酸
油酸
油菜籽
化学
脂肪酶
亚油酸
食品科学
水解
脂肪酸
生物化学
三烯丙基
色谱法
有机化学
酶
作者
Xi Cao,Yayu Pan,Miao Qiao,Yongjun Yuan
出处
期刊:Food Chemistry
[Elsevier]
日期:2022-04-06
卷期号:387: 132907-132907
被引量:13
标识
DOI:10.1016/j.foodchem.2022.132907
摘要
Rapeseed oil has a similar oleic acid/linoleic acid ratio to human milk fat (HMF). However, it can hardly be used for human milk fat substitute (HMFS) synthesis due to high erucic acid content. In this study, Candida cylindracea lipase (CCL) was found to strongly discriminate against erucic acid. Free fatty acids containing low erucic acid and high oleic acid and linoleic acid were prepared from rapeseed oil hydrolysis catalyzed by CCL. The erucic acid content was only 1.58% (initial 8.70%), when the degree of hydrolysis reached 79.58%. The free fatty acids were used as acyl-donors in the acidolysis catalyzed by Novozym 40086. Considering acyl incorporation and migration, the optimum conditions were 1:8 (tripalmitin to acyl-donors), 40 °C and 2 h. The erucic acid content dropped to 0.97% in the HMFS. According to the Q-TOF-MS analysis, the HMFS was rich in 1,3-dioleoyl-2-palmitoyl-glycerol (18.20%) and 1-oleoyl-2-palmitoyl-3-linoleoyl-glycerol (17.96%), which was similar to HMF.
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