Fully Automated Chemiluminescence Microarray Analysis Platform for Rapid and Multiplexed SARS-CoV-2 Serodiagnostics

免疫分析 分析物 化学发光 微阵列 抗原 抗体 计算生物学 化学 免疫学 色谱法 生物 生物化学 基因表达 基因
作者
Julia Klüpfel,Sandra Paßreiter,Nina Weidlein,Martin Knopp,Martin Ungerer,Ulrike Protzer,Percy A. Knolle,Oliver Hayden,Martin Elsner,Michael Seidel
出处
期刊:Analytical Chemistry [American Chemical Society]
卷期号:94 (6): 2855-2864 被引量:6
标识
DOI:10.1021/acs.analchem.1c04672
摘要

Lateral-flow immunoassays and laboratory diagnostic tests like enzyme-linked immunosorbent assays (ELISAs) are powerful diagnostic tools to help fight the COVID-19 pandemic using them as antigen or antibody tests. However, the need emerges for alternative bioanalytical systems that combine their favorable features─simple, rapid, and cost-efficient point-of-care (POC) analysis of lateral-flow immunoassays and higher reliability of laboratory tests─while eliminating their disadvantages (limited sensitivity and specificity of lateral-flow assays and prolonged time and work expenditure of laboratory analysis). An additional need met by only a few tests is multiplexing, allowing for the analysis of several immunorecognition patterns at the same time. We herein present a strategy to combine all desirable attributes of the different test types by means of a flow-based chemiluminescence microarray immunoassay. Laminated polycarbonate microarray chips were developed for easy production and subsequent application in the fully automated microarray analysis platform MCR-R, where a novel flow cell design minimizes the sample volume to 40 μL. This system was capable of detecting IgG antibodies to SARS-CoV-2 with 100% sensitivity and specificity using recombinant antigens for the SARS-CoV-2 spike S1 protein, nucleocapsid protein, and receptor binding domain. The analysis was accomplished within under 4 min from serum, plasma, and whole blood, making it also useful in POC settings. Additionally, we showed the possibility of serosurveillance after infection or vaccination to monitor formerly unnoticed breakthrough infections in the population as well as to detect the need for booster vaccination after the natural decline of the antibody titer below detectable levels. This will help in answering pressing questions on the importance of the antibody response to SARS-CoV-2 that so far remain open. Additionally, even the sequential detection of IgM and IgG antibodies was possible, allowing for statements on the time response of an infection. While our serodiagnostic application focuses on SARS-CoV-2, the same approach is easily adjusted to other diseases, making it a powerful tool for future serological testing.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
烟花应助王走走采纳,获得10
刚刚
wenqin发布了新的文献求助10
刚刚
2秒前
Tony完成签到,获得积分10
2秒前
3秒前
aaa发布了新的文献求助10
4秒前
青岚完成签到,获得积分10
4秒前
xin发布了新的文献求助10
6秒前
7秒前
9秒前
10秒前
呐呐呐呐呐呐完成签到 ,获得积分20
14秒前
15秒前
guoxingliu完成签到,获得积分10
15秒前
踏实的鸽子完成签到,获得积分10
15秒前
16秒前
gstaihn发布了新的文献求助10
18秒前
星辰大海应助殷勤的惜寒采纳,获得10
18秒前
脑洞疼应助武雨寒采纳,获得10
19秒前
小巫发布了新的文献求助10
21秒前
楚子航发布了新的文献求助10
22秒前
闪闪完成签到,获得积分20
22秒前
慕青应助任性半凡采纳,获得10
22秒前
秀丽大凄发布了新的文献求助30
23秒前
三土完成签到,获得积分10
26秒前
小巫完成签到,获得积分10
26秒前
星辰大海应助科研通管家采纳,获得10
28秒前
Akim应助科研通管家采纳,获得10
28秒前
敬老院N号应助科研通管家采纳,获得30
29秒前
赘婿应助科研通管家采纳,获得10
29秒前
29秒前
bkagyin应助科研通管家采纳,获得10
29秒前
njr完成签到,获得积分10
29秒前
29秒前
29秒前
30秒前
闪闪发布了新的文献求助10
33秒前
Ammon发布了新的文献求助10
36秒前
温柔孤兰发布了新的文献求助10
37秒前
金东华完成签到,获得积分10
37秒前
高分求助中
The late Devonian Standard Conodont Zonation 2000
Nickel superalloy market size, share, growth, trends, and forecast 2023-2030 2000
The Lali Section: An Excellent Reference Section for Upper - Devonian in South China 1500
Very-high-order BVD Schemes Using β-variable THINC Method 870
Mantiden: Faszinierende Lauerjäger Faszinierende Lauerjäger 800
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 800
Fundamentals of Dispersed Multiphase Flows 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3254356
求助须知:如何正确求助?哪些是违规求助? 2896550
关于积分的说明 8293206
捐赠科研通 2565501
什么是DOI,文献DOI怎么找? 1393074
科研通“疑难数据库(出版商)”最低求助积分说明 652418
邀请新用户注册赠送积分活动 629946