In this study, we investigated the macro and micro mechanisms of bamboo bending failure, and a new method to unfasten bamboo fiber by continuous bends and large deformations is put forward. The method could effectively maintain the fiber structure integrity without damage. Bamboo strip with load deformation was considered a continuous beam, and the bending moment and the matrix cracking of the bamboo were analyzed on the basis of elastically plastic theory. The mechanical model to unfasten the bamboo fiber under continuous bending was established using the principle of virtual work, while the loading conditions were obtained. The arithmetic expressions for the loading roller diameter and distance, as the main parameters, were obtained analyzing bamboo splitting under different strain conditions. The fiber splitting test further verifies our method and models, which provide a new idea and way for bamboo fiber splitting by mechanical method.